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Effect of plasma-nitrided titanium surfaces on the differentiation of pre-osteoblastic cells.
Moura, Carlos Eduardo B; Queiroz Neto, Moacir F; Braz, Janine Karla F S; de Medeiros Aires, Michelle; Silva Farias, Naisandra B; Barboza, Carlos Augusto G; Cavalcanti Júnior, Geraldo B; Rocha, Hugo Alexandre O; Alves Junior, Clodomiro.
Afiliação
  • Moura CEB; Department of Animal Sciences, Federal Rural University of Semiarid Region (UFERSA), Mossoró, Brazil.
  • Queiroz Neto MF; Department of Animal Sciences, Federal Rural University of Semiarid Region (UFERSA), Mossoró, Brazil.
  • Braz JKFS; Department of Animal Sciences, Federal Rural University of Semiarid Region (UFERSA), Mossoró, Brazil.
  • de Medeiros Aires M; Department of Animal Sciences, Federal Rural University of Semiarid Region (UFERSA), Mossoró, Brazil.
  • Silva Farias NB; Department of Animal Sciences, Federal Rural University of Semiarid Region (UFERSA), Mossoró, Brazil.
  • Barboza CAG; Department of Animal Sciences, Federal Rural University of Semiarid Region (UFERSA), Mossoró, Brazil.
  • Cavalcanti Júnior GB; Department of Animal Sciences, Federal Rural University of Semiarid Region (UFERSA), Mossoró, Brazil.
  • Rocha HAO; Department of Animal Sciences, Federal Rural University of Semiarid Region (UFERSA), Mossoró, Brazil.
  • Alves Junior C; Department of Animal Sciences, Federal Rural University of Semiarid Region (UFERSA), Mossoró, Brazil.
Artif Organs ; 43(8): 764-772, 2019 Aug.
Article em En | MEDLINE | ID: mdl-30779451
A titanium surface nitrided by plasma contains nitrogen ions that guarantee resistance to corrosion and biocompatibility. Despite this, no descriptions concerning the influence of the expression of cell adhesion proteins and their influence on osteogenic cell differentiation are available. Thus, the present study aimed to assess the response of murine pre-osteoblastic cells (MC3T3-E1) cultured on nitrided titanium surfaces. Pre-osteoblastic cells were grown on polished titanium discs, used as controls, and on previously characterized plasma-nitrided titanium discs. Cells from both groups were submitted to the MTT cell viability test. The expressions of α5, α2, and ß1 integrin were assessed by flow cytometry and immunofluorescence, while osteocalcin expression was assessed by flow cytometry. The nitrided surface presented higher α2 and ß1 integrin expressions, as well as osteocalcin expression, when compared to the polished surface, with no alterations in cell viability. These findings seem to suggest that the plasma nitriding treatment produces a titanium surface with the potential for effective in vitro osseointegration.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Osteoblastos / Titânio / Materiais Biocompatíveis / Gases em Plasma Limite: Animals Idioma: En Revista: Artif Organs Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Osteoblastos / Titânio / Materiais Biocompatíveis / Gases em Plasma Limite: Animals Idioma: En Revista: Artif Organs Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos