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A new immunoassay of hybrid nanomater conjugated to aptamers for the detection of dengue virus.
Basso, Caroline R; Crulhas, Bruno P; Magro, Massimiliano; Vianello, Fabio; Pedrosa, Valber A.
Afiliação
  • Basso CR; Institute of Bioscience, Department of Chemistry and Biochemistry, UNESP, Botucatu, SP, Brazil.
  • Crulhas BP; Institute of Bioscience, Department of Chemistry and Biochemistry, UNESP, Botucatu, SP, Brazil.
  • Magro M; Department of Comparative Biomedicine and Food Science, University of Padua, 35020 Legnaro, Italy.
  • Vianello F; Department of Comparative Biomedicine and Food Science, University of Padua, 35020 Legnaro, Italy.
  • Pedrosa VA; Institute of Bioscience, Department of Chemistry and Biochemistry, UNESP, Botucatu, SP, Brazil. Electronic address: vpedrosa@ibb.unesp.br.
Talanta ; 197: 482-490, 2019 May 15.
Article em En | MEDLINE | ID: mdl-30771965
A new immunosensor using hybrid nanomaterials for the detection of dengue virus was demonstrated in this work. This immunosensor composed of nanoparticles of γ-Fe2O3(SAMN) modified with MPA- SAMN@MPA was characterized by FTIR spectroscopy, transmission electron microscopy,quartz crystal microbalance, UV-vis and LSPR technique. The binding of SAMN@MPA with AuNPs conjugated with aptamers(SAMN@MPA@AuNPs@aptamer) provides specific chemical bonds to four dengue serotypes. Colorimetric changes in the modification steps provided rapid visual detection of the virus without the use of equipment. Variations of aptamers concentrations 1.0-10.0 µM where the 3.0 µM aptamer concentration is sufficient to completely cover the surface of the modified AuNPs with an R2 value of> 0.99. This new proposed methodology presenting some advantages in relation to traditional detection methods such as time optimization and cost,can be used as a diagnostic method.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Imunoensaio / Vírus da Dengue / Óxido Ferroso-Férrico / Aptâmeros de Nucleotídeos / Nanopartículas de Magnetita Tipo de estudo: Diagnostic_studies Idioma: En Revista: Talanta Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Imunoensaio / Vírus da Dengue / Óxido Ferroso-Férrico / Aptâmeros de Nucleotídeos / Nanopartículas de Magnetita Tipo de estudo: Diagnostic_studies Idioma: En Revista: Talanta Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda