Your browser doesn't support javascript.
loading
High BAFF expression associated with active disease in systemic lupus erythematosus and relationship with rs9514828C>T polymorphism in TNFSF13B gene.
Marín-Rosales, M; Cruz, A; Salazar-Camarena, D C; Santillán-López, E; Espinoza-García, N; Muñoz-Valle, J F; Ramírez-Dueñas, M G; Oregón-Romero, E; Orozco-Barocio, G; Palafox-Sánchez, C A.
Afiliação
  • Marín-Rosales M; Research Institute of Biomedical Sciences (IICB), University Center for Health Sciences, University of Guadalajara, Sierra Mojada 950, Edificio Q, Primer Piso, CP 44340, Guadalajara, Jalisco, Mexico.
  • Cruz A; Biomedical Sciences, University Center for Health Sciences, University of Guadalajara, Guadalajara, Mexico.
  • Salazar-Camarena DC; Department of Rheumatology, West General Hospital, Ministry of Health, Guadalajara, Mexico.
  • Santillán-López E; Research Institute of Biomedical Sciences (IICB), University Center for Health Sciences, University of Guadalajara, Sierra Mojada 950, Edificio Q, Primer Piso, CP 44340, Guadalajara, Jalisco, Mexico.
  • Espinoza-García N; Research Institute of Biomedical Sciences (IICB), University Center for Health Sciences, University of Guadalajara, Sierra Mojada 950, Edificio Q, Primer Piso, CP 44340, Guadalajara, Jalisco, Mexico.
  • Muñoz-Valle JF; Research Institute of Biomedical Sciences (IICB), University Center for Health Sciences, University of Guadalajara, Sierra Mojada 950, Edificio Q, Primer Piso, CP 44340, Guadalajara, Jalisco, Mexico.
  • Ramírez-Dueñas MG; Biomedical Sciences, University Center for Health Sciences, University of Guadalajara, Guadalajara, Mexico.
  • Oregón-Romero E; Research Institute of Biomedical Sciences (IICB), University Center for Health Sciences, University of Guadalajara, Sierra Mojada 950, Edificio Q, Primer Piso, CP 44340, Guadalajara, Jalisco, Mexico.
  • Orozco-Barocio G; Research Institute of Biomedical Sciences (IICB), University Center for Health Sciences, University of Guadalajara, Sierra Mojada 950, Edificio Q, Primer Piso, CP 44340, Guadalajara, Jalisco, Mexico.
  • Palafox-Sánchez CA; Department of Physiology, Immunology Laboratory, University Center for Health Sciences, University of Guadalajara, Guadalajara, Mexico.
Clin Exp Med ; 19(2): 183-190, 2019 May.
Article em En | MEDLINE | ID: mdl-30747361
B cell-activating factor (BAFF) promotes the survival, proliferation and maturation of B lymphocytes, which are key elements in the pathogenesis of systemic lupus erythematosus (SLE). This cytokine is encoded on TNFSF13B gene, and diverse single-nucleotide polymorphisms have been associated with susceptibility in different autoimmune disorders. In this study, the relationship of TNFSF13B gene rs9514827T>C, rs1041567T>A and rs9514828C>T polymorphisms, mRNA expression and soluble BAFF levels was investigated in 175 SLE patients and 208 healthy controls (HC). The TNFSF13B polymorphisms were evaluated by PCR-RFLP technique. The TNFSF13B gene expression was quantified through the RT-PCR assays. The soluble BAFF (sBAFF) levels were measured with ELISA test. There were no differences in genotype and allele frequencies for the three TNFSF13B polymorphisms, between SLE patients and HC. SLE patients showed 3.15-fold more TNFSF13B gene expression than HC. The patients who displayed most mRNA expression were those with active disease and the carriers of rs9514828 T variant allele. The sBAFF serum levels were higher in SLE patients compared to HC (2.083 vs. 0.742 ng/mL, p < 0.001). The SLE patients with active disease showed the higher sBAFF serum levels (2.403 ng/mL), mainly patients with lupus nephritis and hematological manifestations. In addition, a correlation of sBAFF with disease activity was found (r = 0.32, p < 0.001). In conclusion, the TNFSF13B gene polymorphisms were not found to be associated with SLE susceptibility in Mexican mestizos. Nevertheless, rs9514828C>T polymorphism seems to increase TNFSF13B gene expression. High BAFF expression is related to active disease, renal and hematological involvement; therefore, it could be considered as follow-up biomarker in SLE patients.
Assuntos
Palavras-chave
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Expressão Gênica / Predisposição Genética para Doença / Polimorfismo de Nucleotídeo Único / Fator Ativador de Células B / Lúpus Eritematoso Sistêmico Tipo de estudo: Risk_factors_studies Limite: Adolescent / Adult / Female / Humans País/Região como assunto: Mexico Idioma: En Revista: Clin Exp Med Assunto da revista: MEDICINA Ano de publicação: 2019 Tipo de documento: Article País de afiliação: México País de publicação: Itália
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Expressão Gênica / Predisposição Genética para Doença / Polimorfismo de Nucleotídeo Único / Fator Ativador de Células B / Lúpus Eritematoso Sistêmico Tipo de estudo: Risk_factors_studies Limite: Adolescent / Adult / Female / Humans País/Região como assunto: Mexico Idioma: En Revista: Clin Exp Med Assunto da revista: MEDICINA Ano de publicação: 2019 Tipo de documento: Article País de afiliação: México País de publicação: Itália