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Diethyldithiocarbamate loaded in beeswax-copaiba oil nanoparticles obtained by solventless double emulsion technique promote promastigote death in vitro.
Mazur, Karin Luize; Feuser, Paulo Emílio; Valério, Alexsandra; Poester Cordeiro, Arthur; de Oliveira, Camila Indiani; Assolini, João Paulo; Pavanelli, Wander Rogério; Sayer, Claudia; Araújo, Pedro H H.
Afiliação
  • Mazur KL; Department of Chemical Engineering and Food Engineering, Federal University of Santa Catarina, Brazil.
  • Feuser PE; Department of Chemical Engineering and Food Engineering, Federal University of Santa Catarina, Brazil.
  • Valério A; Department of Chemical Engineering and Food Engineering, Federal University of Santa Catarina, Brazil.
  • Poester Cordeiro A; Department of Chemical Engineering and Food Engineering, Federal University of Santa Catarina, Brazil.
  • de Oliveira CI; Instituto Gonçalo Moniz, Fundação Oswaldo Cruz (FIOCRUZ), Salvador, Brazil.
  • Assolini JP; Laboratory of Experimental Protozoology, Department of Pathological Sciences, Center of Biological Sciences, State University of Londrina, PR, Brazil.
  • Pavanelli WR; Laboratory of Experimental Protozoology, Department of Pathological Sciences, Center of Biological Sciences, State University of Londrina, PR, Brazil.
  • Sayer C; Department of Chemical Engineering and Food Engineering, Federal University of Santa Catarina, Brazil.
  • Araújo PHH; Department of Chemical Engineering and Food Engineering, Federal University of Santa Catarina, Brazil. Electronic address: pedro.h.araujo@ufsc.br.
Colloids Surf B Biointerfaces ; 176: 507-512, 2019 Apr 01.
Article em En | MEDLINE | ID: mdl-30711703
Leishmaniasis is considered a neglected tropical disease that represents a Public Health problem due to its high incidence. In the search of new alternatives for Leishmaniasis treatment diethyldithiocarbamate (DETC) has shown an excellent leishmanicidal activity and the incorporation into drug carrier systems, such as solid lipid nanoparticles (SLNs), is very promising. In the present work DETC loaded in beeswax nanoparticles containing copaiba oil were obtained by the double emulsion/melt technique. The nanoparticles were characterized and leishmanicidal activity against L. amazonensis promastigotes forms and cytotoxicity in murine macrophages were evaluated. SLNs presented size below 200 nm, spherical morphology, negative charge surface, high encapsulation efficiency, above 80%, and excellent stability. Moreover, Fourier transform infrared spectroscopy (FTIR) and differential scanning calorimetry (DSC) analyses were performed to evaluate the chemical structure and possible interactions between DETC and SLNs. SLNs provided a protection for DETC, decreasing its cytotoxic effects in macrophages, which led to an improvement in the selectivity against the parasites, which almost doubled from free DETC (11.4) to DETC incorporated in SLNs (18.2). These results demonstrated that SLNs had a direct effect on L. amazonensis promastigotes without affect the viability of macrophage cell, can be a promising alternative therapy for the cutaneous treatment of L. amazonensis.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ceras / Óleos de Plantas / Ditiocarb / Emulsões / Nanopartículas / Fabaceae / Leishmania Limite: Animals Idioma: En Revista: Colloids Surf B Biointerfaces Assunto da revista: QUIMICA Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ceras / Óleos de Plantas / Ditiocarb / Emulsões / Nanopartículas / Fabaceae / Leishmania Limite: Animals Idioma: En Revista: Colloids Surf B Biointerfaces Assunto da revista: QUIMICA Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda