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A Survey on Occurrence of Cladosporium fulvum Identifies Race 0 and Race 2 in Tomato-Growing Areas of Argentina.
Medina, Rocío; López, Silvina M Y; Franco, Mario E E; Rollan, Cristina; Ronco, Blanca L; Saparrat, Mario C N; De Wit, Pierre J G M; Balatti, Pedro A.
Afiliação
  • Medina R; Centro de Investigaciones en Fitopatologías (CIDEFI), La Plata (1900), Argentina; and Centro de Investigación y Desarrollo en Fermentaciones Industriales (CINDEFI), La Plata (1900), Argentina.
  • López SMY; Instituto de Fisiología Vegetal (INFIVE), La Plata (1900), Argentina.
  • Franco MEE; CIDEFI, La Plata (1900), Argentina.
  • Rollan C; CIDEFI, La Plata (1900), Argentina.
  • Ronco BL; CIDEFI, La Plata (1900), Argentina.
  • Saparrat MCN; INFIVE, La Plata (1900), Argentina.
  • De Wit PJGM; Wageningen University, Laboratory of Phytopathology, Wageningen, The Netherlands.
  • Balatti PA; CIDEFI, La Plata (1900), Argentina; and Comisión de Investigaciones Científicas de la Provincia de Buenos Aires, Argentina.
Plant Dis ; 99(12): 1732-1737, 2015 Dec.
Article em En | MEDLINE | ID: mdl-30699511
The presence of Cladosporium fulvum (syn. Passalora fulva), causal agent of tomato leaf mold, was confirmed in the two main greenhouse-production areas for tomato in Argentina. Using both morphological characters and internal transcribed spacer sequencing, we confirmed the presence of physiological races of this pathogen. A diagnostic multiplex polymerase chain reaction (PCR) was also developed, using primers derived from C. fulvum avirulence (Avr) genes. In all, 20 isolates of Cladosporium spp. were obtained as monospore cultures and 12 were identified as C. fulvum. By this method, we showed that, of these 12 isolates, 5 were race 0 (carrying functional Avr2, Avr4, Avr4E, and Avr9 genes) and 7 were race 2 (lacking the Avr2 gene). Race identity was confirmed by testing their virulence on a set of tomato differentials carrying different Cf resistance genes. All Avr genes could be amplified in single or multiplex PCR using DNA isolated from in vitro grown monospore cultures but only three Avr could be amplified when genomic DNA was isolated from C. fulvum-infected necrotic leaf tissue.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Aspecto: Determinantes_sociais_saude País/Região como assunto: America do sul / Argentina Idioma: En Revista: Plant Dis Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Argentina País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Aspecto: Determinantes_sociais_saude País/Região como assunto: America do sul / Argentina Idioma: En Revista: Plant Dis Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Argentina País de publicação: Estados Unidos