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Genotypic and Phenotypic Characterization of Streptomyces Species Causing Potato Common Scab in Uruguay.
Lapaz, M I; Huguet-Tapia, J C; Siri, M I; Verdier, E; Loria, R; Pianzzola, M J.
Afiliação
  • Lapaz MI; Cátedra de Microbiología, Departamento de Biociencias, Facultad de Química (FQ), Universidad de la República, 11800 Montevideo, Uruguay.
  • Huguet-Tapia JC; Department of Plant Pathology, Institute of Food and Agricultural Sciences, University of Florida (UF-IFAS), Gainesville 32611 USA.
  • Siri MI; Cátedra de Microbiología, Departamento de Biociencias, FQ, Universidad de la República, 11800 Montevideo, Uruguay.
  • Verdier E; Dirección General de Servicios Agrícolas, Departamento Laboratorios Biológicos, Ministerio de Ganadería Agricultura y Pesca, 12900 Montevideo, Uruguay.
  • Loria R; Department of Plant Pathology, UF-IFAS, Gainesville 32611 USA.
  • Pianzzola MJ; Cátedra de Microbiología, Departamento de Biociencias, FQ, Universidad de la República, 11800 Montevideo, Uruguay.
Plant Dis ; 101(8): 1362-1372, 2017 Aug.
Article em En | MEDLINE | ID: mdl-30678602
Isolation and characterization of common scab (CS) pathogen Streptomyces spp. from Uruguayan potato tubers and soil samples were done in response to significant economic losses due to CS on potato in autumn 2010. Seventy of the 331 isolates were classified as pathogenic owing to their ability to induce necrosis on tuber disks and stunting of radish seedling. Streptomyces spp. causing CS on potato in Uruguay were found to represent a range of different species by virtue of their diverse morphological and physiological traits as well as rep-PCR, rpoB phylogenetic analysis, and multi-locus sequences analysis. We identified isolates primarily as Streptomyces scabiei, S. acidiscabies, and S. europaeiscabiei. However, some of the pathogenic isolates still remain to be identified at the species level. This highlights the need for improved methods for discrimination among pathogenic Streptomyces species. The presence of Streptomyces pathogenicity island (PAI) genes was analyzed, including genes encoding for thaxtomin synthetase (txtA, txtB), tomatinase (tomA), and a necrosis protein (nec1). Among the isolates that were pathogenic, 50% contained the four pathogenicity genes, 33% had an atypical composition of PAI marker genes, and 17% did not contain any genes. The absence of the genes reported to be involved in thaxtomin biosynthesis (txtA, txtB) was confirmed by whole-genome sequencing of two representative strains of this group. This finding suggests the participation of other virulence factors in plant pathogenicity.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Streptomyces / Solanum tuberosum Tipo de estudo: Prognostic_studies País/Região como assunto: America do sul / Uruguay Idioma: En Revista: Plant Dis Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Uruguai País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Streptomyces / Solanum tuberosum Tipo de estudo: Prognostic_studies País/Região como assunto: America do sul / Uruguay Idioma: En Revista: Plant Dis Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Uruguai País de publicação: Estados Unidos