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Label-free quantitative proteomic analysis reveals potential biomarkers for early healing in cutaneous leishmaniasis.
Montoya, Andrés; López, Manuel Carlos; Vélez, Ivan D; Robledo, Sara M.
Afiliação
  • Montoya A; PECET, Facultad de Medicina, Universidad de Antioquia, Medellin, Antioquia, Colombia.
  • López MC; Molecular Biology Department Consejo Superior de Investigaciones Científicas, Instituto de Parasitología y Biomedicina "López Neyra", Granade, Spain.
  • Vélez ID; PECET, Facultad de Medicina, Universidad de Antioquia, Medellin, Antioquia, Colombia.
  • Robledo SM; PECET, Facultad de Medicina, Universidad de Antioquia, Medellin, Antioquia, Colombia.
PeerJ ; 6: e6228, 2019.
Article em En | MEDLINE | ID: mdl-30648003
BACKGROUND: Leishmaniasis is a parasitic disease caused by more than 20 species of the Leishmania genus. The disease is globally distributed and is endemic in 97 countries and three territories in the tropical and subtropical regions. The efficacy of the current treatments is becoming increasingly low either due to incomplete treatment or resistant parasites. Failure of treatment is frequent, and therefore, the search for early biomarkers of therapeutic response in cutaneous leishmaniasis (CL) is urgently needed. OBJECTIVE: The aim of this study was to compare the proteomic profiles in patients with CL before and after 7 days of treatment and identify early biomarkers of curative response. METHODS: Four patients with a parasitological diagnosis of leishmaniasis with confirmation of species by PCR-RFLP were recruited. All patients had a single lesion, and a protein from the middle of the ulcer was quantified by liquid chromatography and mass spectrometry. RESULTS: A total of 12 proteins showed differential expression in the comparative LC-electrospray ionization MS/MS (LC-ESI-MS/MS) triplicate analysis. Seven of them were up-regulated and five of them were down-regulated. Calcium binding proteins A2, A8, and A9 and hemoglobin subunits alpha-2 and delta showed high correlation with epidermis development and immune response. CONCLUSION: We identified changes in the profiles of proteins that had a positive therapeutic response to the treatment. The proteins identified with differential expression are related to the reduction of inflammation and increased tissue repair. These proteins can be useful as biomarkers for early monitoring of therapeutic response in CL.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: PeerJ Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Colômbia País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: PeerJ Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Colômbia País de publicação: Estados Unidos