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PTPN22 +788 G>A (R263Q) Polymorphism is Associated with mRNA Expression but it is not a Susceptibility Marker for Rheumatoid Arthritis Patients from Western Mexico.
Ramírez-Pérez, S; Sánchez-Zuno, G A; Chavarría-Buenrostro, L E; Montoya-Buelna, M; Reyes-Pérez, I V; Ramírez-Dueñas, M G; Palafox-Sánchez, C A; Martínez-Bonilla, G E; Muñoz-Valle, J F.
Afiliação
  • Ramírez-Pérez S; Research Institute in Biomedical Sciences, University Center of Health Science, University of Guadalajara, Guadalajara, Jalisco, Mexico.
  • Sánchez-Zuno GA; Transdisciplinary Institute of Research and Services (ITRANS), University of Guadalajara, Zapopan, Jalisco, Mexico.
  • Chavarría-Buenrostro LE; Research Institute in Biomedical Sciences, University Center of Health Science, University of Guadalajara, Guadalajara, Jalisco, Mexico.
  • Montoya-Buelna M; Transdisciplinary Institute of Research and Services (ITRANS), University of Guadalajara, Zapopan, Jalisco, Mexico.
  • Reyes-Pérez IV; Immunology Laboratory, Department of Physiology, University Center of Health Science, University of Guadalajara, Guadalajara, Jalisco, Mexico.
  • Ramírez-Dueñas MG; Immunology Laboratory, Department of Physiology, University Center of Health Science, University of Guadalajara, Guadalajara, Jalisco, Mexico.
  • Palafox-Sánchez CA; Immunology Laboratory, Department of Physiology, University Center of Health Science, University of Guadalajara, Guadalajara, Jalisco, Mexico.
  • Martínez-Bonilla GE; Research Institute in Biomedical Sciences, University Center of Health Science, University of Guadalajara, Guadalajara, Jalisco, Mexico.
  • Muñoz-Valle JF; Immunology Laboratory, Department of Physiology, University Center of Health Science, University of Guadalajara, Guadalajara, Jalisco, Mexico.
Biochem Genet ; 57(3): 455-465, 2019 Jun.
Article em En | MEDLINE | ID: mdl-30637604
PTPN22 represents an important non-HLA gene that has been strongly associated with rheumatoid arthritis (RA) pathogenesis. Several studies have reported a specific genetic variant for PTPN22 (+788 G>A; rs33996649) that might be associated with decreased RA risk in Caucasian population; nevertheless, its specific role in western Mexican population has not been yet described. A case-control study with 443 RA patients and 317 control subjects (CS) was conducted. The genotyping was performed by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) technique and the PTPN22 mRNA expression was determined by SYBR Green-based real-time quantitative-PCR assay. No association between the PTPN22 +788 G>A polymorphism and RA susceptibility in western Mexican population was found when comparing genotype and allelic frequencies between RA patients and CS (G/G vs. G/A: OR 0.55, p = 0.14, 95% CI 0.22-1.32; G vs. A: OR 0.56, p = 0.14, 95% CI 0.23-1.36). The PTPN22 mRNA expression increased 4.6-fold more in RA patients than in CS, and RA patients, carriers of PTPN22 +788 G/A genotype, expressed 15.6-fold more than RA patients carrying the homozygous G/G genotype. Overall, these results showed that the PTPN22 +788 G>A polymorphism is not associated with RA susceptibility in western Mexican population, whereas the presence of G/A genotype is associated with increased PTPN22 mRNA expression in RA patients.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Artrite Reumatoide / Polimorfismo de Fragmento de Restrição / Marcadores Genéticos / Predisposição Genética para Doença / Proteína Tirosina Fosfatase não Receptora Tipo 22 Tipo de estudo: Observational_studies / Risk_factors_studies Limite: Adult / Aged / Female / Humans / Male / Middle aged País/Região como assunto: Mexico Idioma: En Revista: Biochem Genet Ano de publicação: 2019 Tipo de documento: Article País de afiliação: México País de publicação: Estados Unidos
Texto completo
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XML
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Artrite Reumatoide / Polimorfismo de Fragmento de Restrição / Marcadores Genéticos / Predisposição Genética para Doença / Proteína Tirosina Fosfatase não Receptora Tipo 22 Tipo de estudo: Observational_studies / Risk_factors_studies Limite: Adult / Aged / Female / Humans / Male / Middle aged País/Região como assunto: Mexico Idioma: En Revista: Biochem Genet Ano de publicação: 2019 Tipo de documento: Article País de afiliação: México País de publicação: Estados Unidos