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Selection of Saccharomyces cerevisiae isolates for ethanol production in the presence of inhibitors.
Cabañas, Keyla Tortoló; Peña-Moreno, Irina Charlot; Parente, Denise Castro; García, Antonio Bell; Gutiérrez, Roxana García; de Morais, Marcos Antonio.
Afiliação
  • Cabañas KT; 1Department of Alcohol, Yeasts and Animal Food, Cuban Research Institute of Sugar Cane Byproducts (ICIDCA), San Miguel del Padrón-11 000, P. O. Box 4026, La Habana, Cuba.
  • Peña-Moreno IC; 3Department of Genetics, Federal University of Pernambuco (UFPE), Av. Moraes Rego, 1235 Cidade Universitária, 50670-901 Recife, PE Brazil.
  • Parente DC; 3Department of Genetics, Federal University of Pernambuco (UFPE), Av. Moraes Rego, 1235 Cidade Universitária, 50670-901 Recife, PE Brazil.
  • García AB; 1Department of Alcohol, Yeasts and Animal Food, Cuban Research Institute of Sugar Cane Byproducts (ICIDCA), San Miguel del Padrón-11 000, P. O. Box 4026, La Habana, Cuba.
  • Gutiérrez RG; 2Department of Microbiology, Cuban Research Institute of Sugar Cane Byproducts (ICIDCA), San Miguel del Padrón-11 000, P. O. Box 4026, La Habana, Cuba.
  • de Morais MA; 3Department of Genetics, Federal University of Pernambuco (UFPE), Av. Moraes Rego, 1235 Cidade Universitária, 50670-901 Recife, PE Brazil.
3 Biotech ; 9(1): 6, 2019 Jan.
Article em En | MEDLINE | ID: mdl-30622844
Eight yeast isolates identified as Saccharomyces cerevisiae were recovered from molasses-using Cuban distilleries and discriminated by nucleotide sequence analysis of ITS locus. The isolates L/25-7-81 and L/25-7-86 showed the highest ethanol yield from sugarcane juice, while L/25-7-12 and L/25-7-79 showed high ethanol yield from sugarcane molasses. The isolate L/25-7-86 also displayed high fermentation capacity when molasses was diluted with vinasse. In addition, stress tolerance was evaluated on the basis of growth in the presence of inhibitors (acetic acid, lactic acid, 5-hydroxymethylfurfural and sulfuric acid) and the results indicated that L/25-7-77 and L/25-7-79 congregated the highest score for cross-tolerance and fermentation capacity. Hence, these isolates, especially L/25-7-77, could serve as potential biological platform for the arduous task of fermenting complex substrates that contain inhibitors. The use of these yeasts was discussed in the context of second-generation ethanol and the environmental and economic implications of the use of vinasse, saving the use of water for substrate dilution.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Prognostic_studies Idioma: En Revista: 3 Biotech Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Cuba País de publicação: Alemanha

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Prognostic_studies Idioma: En Revista: 3 Biotech Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Cuba País de publicação: Alemanha