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Enzymatically cross-linked arabinoxylan microspheres as oral insulin delivery system.
Martínez-López, A L; Carvajal-Millan, E; Sotelo-Cruz, N; Micard, V; Rascón-Chu, A; López-Franco, Y L; Lizardi-Mendoza, J; Canett-Romero, R.
Afiliação
  • Martínez-López AL; Research Center for Food and Development, CIAD, A.C. Carretera a La Victoria Km. 0.6, Hermosillo, Sonora 83304, Mexico.
  • Carvajal-Millan E; Research Center for Food and Development, CIAD, A.C. Carretera a La Victoria Km. 0.6, Hermosillo, Sonora 83304, Mexico. Electronic address: ecarvajal@ciad.mx.
  • Sotelo-Cruz N; University of Sonora, Rosales y Blvd. Luis D. Colosio, Hermosillo, Sonora 83000, Mexico. Electronic address: nsotelo@guymas.uson.mx.
  • Micard V; Montpellier SupAgro-INRA-UM-CIRAD, JRU IATE, 2, Place Pierre Viala, 34060 Montpellier Cedex 01, France. Electronic address: valerie.micard@supagro.fr.
  • Rascón-Chu A; Research Center for Food and Development, CIAD, A.C. Carretera a La Victoria Km. 0.6, Hermosillo, Sonora 83304, Mexico. Electronic address: arascon@ciad.mx.
  • López-Franco YL; Research Center for Food and Development, CIAD, A.C. Carretera a La Victoria Km. 0.6, Hermosillo, Sonora 83304, Mexico. Electronic address: lopezf@ciad.mx.
  • Lizardi-Mendoza J; Research Center for Food and Development, CIAD, A.C. Carretera a La Victoria Km. 0.6, Hermosillo, Sonora 83304, Mexico. Electronic address: jalim@ciad.mx.
  • Canett-Romero R; University of Sonora, Rosales y Blvd. Luis D. Colosio, Hermosillo, Sonora 83000, Mexico. Electronic address: rcanett@guayacon.uson.mx.
Int J Biol Macromol ; 126: 952-959, 2019 Apr 01.
Article em En | MEDLINE | ID: mdl-30584929
Arabinoxylans (AX) microspheres with different insulin/AX mass ratio were prepared by formation of phenoxy radical issued from the ferulic acid by enzymatic oxidation (entrapped in situ of insulin). Phenolic acid content and FT-IR spectrum of unloaded and insulin-loaded AX microspheres revealed that the phenoxy radical issued from the ferulic acid by enzymatic oxidation did not interact covalently with insulin. The microspheres showed a spherical shape, smooth surface and an average diameter of particles of 320 µm. In vitro control release found that AX microspheres minimized the insulin loss in the upper GI tract, retaining high percentage (~75%) of insulin in its matrix. The stability of the secondary structure of insulin was studied by dichroism circular (CD). The CD spectra of insulin released from AX microspheres did not change according to the insulin/AX mass ratio of the microsphere. Significant hypoglycemic effects with improved insulin-relative bioavailability tested on an in vivo murine model revealed the efficacy of these enzymatically cross-linked arabinoxylans microspheres as a new oral insulin carrier.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Xilanos / Sistemas de Liberação de Medicamentos / Reagentes de Ligações Cruzadas / Lacase / Insulina / Microesferas Limite: Animals / Humans / Male Idioma: En Revista: Int J Biol Macromol Ano de publicação: 2019 Tipo de documento: Article País de afiliação: México País de publicação: Holanda
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Xilanos / Sistemas de Liberação de Medicamentos / Reagentes de Ligações Cruzadas / Lacase / Insulina / Microesferas Limite: Animals / Humans / Male Idioma: En Revista: Int J Biol Macromol Ano de publicação: 2019 Tipo de documento: Article País de afiliação: México País de publicação: Holanda