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In vitro study of Withanolide D toxicity on goat preantral follicles and its effects on the cell cycle.
Guerreiro, Denise Damasceno; de Lima, Laritza Ferreira; de Sá, Naiza Arcângela Ribeiro; Tetaping, Gildas Mbemya; Alves, Benner Geraldo; Lobo, Carlos Henrique; Deusdênia Loiola, Otília; Smitz, Johan; de Figueiredo, José Ricardo; Ribeiro Rodrigues, Ana Paula.
Afiliação
  • Guerreiro DD; Faculty of Veterinary Medicine, Laboratory of Manipulation of Oocyte and Preantral Follicles (LAMOFOPA), State University of Ceara, Avenue Silas Munguba 1700, 60740-000, Fortaleza, CE, Brazil.
  • de Lima LF; Faculty of Veterinary Medicine, Laboratory of Manipulation of Oocyte and Preantral Follicles (LAMOFOPA), State University of Ceara, Avenue Silas Munguba 1700, 60740-000, Fortaleza, CE, Brazil.
  • de Sá NAR; Faculty of Veterinary Medicine, Laboratory of Manipulation of Oocyte and Preantral Follicles (LAMOFOPA), State University of Ceara, Avenue Silas Munguba 1700, 60740-000, Fortaleza, CE, Brazil.
  • Tetaping GM; Faculty of Veterinary Medicine, Laboratory of Manipulation of Oocyte and Preantral Follicles (LAMOFOPA), State University of Ceara, Avenue Silas Munguba 1700, 60740-000, Fortaleza, CE, Brazil.
  • Alves BG; Laboratory of Biology of Reproduction, Federal University of Uberlândia, Minas Gerais, Brazil.
  • Lobo CH; Laboratory of Phytochemical Analysis of Medicinal Plants (LAFIPLAN), Brazil.
  • Deusdênia Loiola O; Laboratory of Phytochemical Analysis of Medicinal Plants (LAFIPLAN), Brazil.
  • Smitz J; Follicle Biology Laboratory, Center for Reproductive Medicine, UZ Brussel, Belgium.
  • de Figueiredo JR; Faculty of Veterinary Medicine, Laboratory of Manipulation of Oocyte and Preantral Follicles (LAMOFOPA), State University of Ceara, Avenue Silas Munguba 1700, 60740-000, Fortaleza, CE, Brazil.
  • Ribeiro Rodrigues AP; Faculty of Veterinary Medicine, Laboratory of Manipulation of Oocyte and Preantral Follicles (LAMOFOPA), State University of Ceara, Avenue Silas Munguba 1700, 60740-000, Fortaleza, CE, Brazil. Electronic address: aprrodriguespapers@gmail.com.
Reprod Toxicol ; 84: 18-25, 2019 03.
Article em En | MEDLINE | ID: mdl-30579997
The Withanolide D is a chemotherapeutic potential against the human tumor cell. However, there is no report on the effect of this compound on ovarian function, especially on preantral folliculogenesis. The aim of this study was to evaluate the toxicity of a new candidate to anticancer drug, Withanolide D (WD) on morphologic integrity, development (activation and granulosa cell proliferation) and gene expression of ABCB1 protein of caprine preantral follicles. Ovarian fragments were cultured in vitro for 2 or 6 days in α-MEM or α-MEM added with paclitaxel (PTX -0.1 µg/mL; negative control) and different concentrations of WD (WD1.5, WD3.0 or WD6.0). The higher dose of WD showed a toxic effect similar to PTX and higher (P < 0.05) than other treatments after 2 and 6 days. In addition, WD6.0 reduced the cell proliferating compared to PTX or mild dose. The expression of ABCB1 remained unchanged in the presence of the chemotherapeutic agents (PTX and WD) throughout the culture period. In conclusion, WD exerted a toxic effect observed by decreasing follicular survival and cell proliferation, on the preantral caprine follicles similar to PTX, whose negative effect on folliculogenesis is already widely known.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Vitanolídeos / Folículo Ovariano / Antineoplásicos Limite: Animals Idioma: En Revista: Reprod Toxicol Assunto da revista: EMBRIOLOGIA / MEDICINA REPRODUTIVA / TOXICOLOGIA Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Vitanolídeos / Folículo Ovariano / Antineoplásicos Limite: Animals Idioma: En Revista: Reprod Toxicol Assunto da revista: EMBRIOLOGIA / MEDICINA REPRODUTIVA / TOXICOLOGIA Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos