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Polymorphism analysis of the apxIA gene of Actinobacillus pleuropneumoniae serovar 5 isolated in swine herds from Brazil.
Dos Santos, Lucas Fernando; Costa Polveiro, Richard; Scatamburlo Moreira, Thalita; Pereira Vidigal, Pedro Marcus; Chang, Yung-Fu; Scatamburlo Moreira, Maria Aparecida.
Afiliação
  • Dos Santos LF; Laboratory of Bacterial Diseases, Sector of Preventive Veterinary Medicine and Public Health, Veterinary Department, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil.
  • Costa Polveiro R; Microbiologia Veterinária Especial LTDA (Microvet), Viçosa, Minas Gerais, Brazil.
  • Scatamburlo Moreira T; Laboratory of Bacterial Diseases, Sector of Preventive Veterinary Medicine and Public Health, Veterinary Department, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil.
  • Pereira Vidigal PM; Laboratory of Bacterial Diseases, Sector of Preventive Veterinary Medicine and Public Health, Veterinary Department, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil.
  • Chang YF; Núcleo de Análise de Biomoléculas (NuBioMol), Center of Biological Sciences, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil.
  • Scatamburlo Moreira MA; Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, New York, United States of America.
PLoS One ; 13(12): e0208789, 2018.
Article em En | MEDLINE | ID: mdl-30562362
The bacterium Actinobacillus pleuropneumoniae is the etiological agent of Contagious Porcine Pleuropneumonia, a disease responsible for economic losses in the swine industry worldwide. A. pleuropneumoniae is capable of producing proteinaceous exotoxins responsible for inducing hemorrhagic lesions, one of which is ApxI. Few studies have conducted an in-depth evaluation of polymorphisms of the nucleotides that make up the ApxI toxin gene. Here we analyze the polymorphisms of the apxIA gene region of A. pleuropneumoniae serovar 5 isolated from swine in different regions in Brazil and report the results of molecular sequencing and phylogenetic analysis. Analysis of the apxIA gene in 60 isolates revealed the presence of genetic diversity and variability. The polymorphisms in the nucleotide sequences determined the grouping of the Brazilian sequences and five more sequences from the GenBank database into 14 different haplotypes, which formed three main groups and revealed the presence of mutations in the nucleotide sequences. The estimation of selection pressures suggests the occurrence of genetic variations by positive selective pressure on A. pleuropneumoniae in large groups of animals in relatively small spaces. These conditions presumably favor the horizontal dissemination of apxIA gene mutations within bacterial populations with host reservoirs. As a result, the same serovar can demonstrate different antigenic capacities due to mutations in the apxIA gene. These alterations in sequences of the apxIA gene could occur in other areas of countries with intense swine production, which could lead to differences in the pathogenicity and immunogenicity of each serovar and have implications for the clinical status or diagnosis of A. pleuropneumoniae.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pleuropneumonia Contagiosa / Polimorfismo Genético / Suínos / Doenças dos Suínos / Acinetobacter / Infecções por Acinetobacter / Genes Bacterianos Limite: Animals País/Região como assunto: America do sul / Brasil Idioma: En Revista: PLoS One Assunto da revista: CIENCIA / MEDICINA Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pleuropneumonia Contagiosa / Polimorfismo Genético / Suínos / Doenças dos Suínos / Acinetobacter / Infecções por Acinetobacter / Genes Bacterianos Limite: Animals País/Região como assunto: America do sul / Brasil Idioma: En Revista: PLoS One Assunto da revista: CIENCIA / MEDICINA Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos