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Mycobacterium "habana" TMC 5135 as a vaccine candidate against tuberculosis: In vitro studies.
MartInez, Jany Vald S; Hernández, Iliana Vald S; Echemendía, Miguel; Rodriguez, Hermis; Yglesias, Arianna; Díaz, Alexis.
Afiliação
  • MartInez JVS; Center for Research, Diagnosis and Reference, CIDR, National Reference Laboratory of Tuberculosis, PAHO/WHO Collaborating Center, Tropical Medicine Institute "Pedro Kouri", Havana, Cuba.
  • Hernández IVS; Center for Research, Diagnosis and Reference, CIDR, National Reference Laboratory of Tuberculosis, PAHO/WHO Collaborating Center, Tropical Medicine Institute "Pedro Kouri", Havana, Cuba.
  • Echemendía M; Center for Research, Diagnosis and Reference, CIDR, National Reference Laboratory of Tuberculosis, PAHO/WHO Collaborating Center, Tropical Medicine Institute "Pedro Kouri", Havana, Cuba.
  • Rodriguez H; Department of Bacteriology Micology, Center for Research, Diagnosis and Reference, CIDR, Cell Culture Laboratory, Tropical Medicine Institute "Pedro Kouri", Havana, Cuba.
  • Yglesias A; Laboratories of Biological-Pharmaceutical Productions (LABIOFAM), Havana, Cuba.
  • Díaz A; Laboratories of Biological-Pharmaceutical Productions (LABIOFAM), Havana, Cuba.
Int J Mycobacteriol ; 7(4): 343-346, 2018.
Article em En | MEDLINE | ID: mdl-30531032
Background: Tuberculosis (TB) is one of the biggest problems of global health, at present. Bacillus-Calmette-Guérin is the only vaccine available against this disease. It protects only against the severe forms of TB in the childhood, which is a challenge in the search of new vaccine candidates. Taking into account the protective history of Mycobacterium "habana" against experimental TB, we proposed to provide the elements that support the use of M. "habana" TMC 5135 as a vaccine candidate against TB by infection studies in murine macrophages cell cultures. Methods: The production of microbicidal compounds dependent on oxygen metabolism as nitric oxide and hydrogen peroxide by murine peritoneal macrophages was detected. The invasive and toxigenic capacity of M. "habana" to infect this cell type was also evaluated through the quantification of intracellular alive bacillus and 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide assay, respectively. Results: The results suggest that M. "habana" TMC 5135 is able to persist into peritoneal macrophages, to resist the effectors mechanisms of respiratory burst, and to keep the viability of the target cell. The demonstration of these effector mechanisms and the survival capacity of M. "habana" in this niche are relevant aspects of this research assuring the continuity of this candidate to next phases of preclinical development. Conclusion: The present investigation contributes to the characterization of the infection by this mycobacteria in its main target cells of innate immunity and it suggest future investigations to evaluate the activation of effector mechanisms of the innate immunity against this candidate.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Tuberculose / Macrófagos Peritoneais / Vacinas contra a Tuberculose Limite: Animals Idioma: En Revista: Int J Mycobacteriol Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Cuba País de publicação: Índia
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Tuberculose / Macrófagos Peritoneais / Vacinas contra a Tuberculose Limite: Animals Idioma: En Revista: Int J Mycobacteriol Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Cuba País de publicação: Índia