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Polymorphisms in the anti-oxidant paraoxonase-1 (PON1) gene associated with fertility of postpartum dairy cows.
Silveira, Pedro Augusto Silva; Butler, W R; LaCount, S E; Overton, T R; Barros, Carlos Castilho; Schneider, Augusto.
Afiliação
  • Silveira PAS; Veterinary College, Universidade Federal de Pelotas, Pelotas, RS, Brazil.
  • Butler WR; Department of Animal Science, Cornell University, Ithaca, NY, 14853, USA.
  • LaCount SE; Department of Animal Science, Cornell University, Ithaca, NY, 14853, USA.
  • Overton TR; Department of Animal Science, Cornell University, Ithaca, NY, 14853, USA.
  • Barros CC; Department of Nutrition, College of Nutrition, Universidade Federal de Pelotas, Pelotas, RS, Brazil.
  • Schneider A; Department of Nutrition, College of Nutrition, Universidade Federal de Pelotas, Pelotas, RS, Brazil. Electronic address: augusto.schneider@ufpel.edu.br.
Theriogenology ; 125: 302-309, 2019 Feb.
Article em En | MEDLINE | ID: mdl-30504072
Paraoxonase 1 (PON1) is a negative acute phase plasma protein synthesized by the liver that has anti-oxidant activity. The aim of this study was to evaluate the association of single nucleotide polymorphisms (SNPs) in the PON1 promoter region with plasma PON1 activity and fertility in Holstein dairy cows. Sixty-eighty Holstein cows were used in this initial investigative study. Blood samples were collected weekly beginning 28 days prior to expected calving, twice weekly in week 1 and 2 postpartum, and then once weekly through 6 weeks postpartum for plasma PON1 activity analysis. Cows were synchronized for ovulation and timed AI at 63-70 DIM using an Ovsynch program. Pregnancy diagnosis was confirmed by rectal palpation and reproductive performance data was recorded until 210 DIM. DNA was extracted from blood of each cow and a fragment of proximal PON1 gene promoter was sequenced. Seven single nucleotide polymorphisms (SNPs) were identified in the promoter region of the PON1 gene at positions -22, -105, -176, -221, -392, -611 and -676, six of which were significantly associated with plasma PON1 activity level. The SNPs -221 and -392 were significantly associated with both plasma PON1 activity and the calving to conception interval (P < 0.05) with no significant effect on calving to first ovulation interval. In conclusion, the genotypes associated with higher plasma PON1 activity in SNP locations -221 and -392 were also associated with a reduced calving to conception interval in this study set of cows. These SNPs may provide novel genetic markers for improved fertility in future larger studies in dairy cows.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Bovinos / Polimorfismo de Nucleotídeo Único / Arildialquilfosfatase / Fertilidade Tipo de estudo: Risk_factors_studies Limite: Animals / Pregnancy Idioma: En Revista: Theriogenology Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Bovinos / Polimorfismo de Nucleotídeo Único / Arildialquilfosfatase / Fertilidade Tipo de estudo: Risk_factors_studies Limite: Animals / Pregnancy Idioma: En Revista: Theriogenology Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos