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Involvement of HbMC1-mediated cell death in tapping panel dryness of rubber tree (Hevea brasiliensis).
Liu, Hui; Wei, Yongxuan; Deng, Zhi; Yang, Hong; Dai, Longjun; Li, Dejun.
Afiliação
  • Liu H; Hainan Provincial Key Laboratory of Tropical Crops Cultivation and Physiology, Key Laboratory of Biology and Genetic Resources of Rubber Tree, Ministry of Agriculture and Rural Affairs, Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou, China.
  • Wei Y; Hainan Provincial Key Laboratory of Tropical Crops Cultivation and Physiology, Key Laboratory of Biology and Genetic Resources of Rubber Tree, Ministry of Agriculture and Rural Affairs, Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou, China.
  • Deng Z; Institute of Tropical Agriculture and Forestry, Hainan University, Haikou, China.
  • Yang H; Hainan Provincial Key Laboratory of Tropical Crops Cultivation and Physiology, Key Laboratory of Biology and Genetic Resources of Rubber Tree, Ministry of Agriculture and Rural Affairs, Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou, China.
  • Dai L; Hainan Provincial Key Laboratory of Tropical Crops Cultivation and Physiology, Key Laboratory of Biology and Genetic Resources of Rubber Tree, Ministry of Agriculture and Rural Affairs, Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou, China.
  • Li D; Hainan Provincial Key Laboratory of Tropical Crops Cultivation and Physiology, Key Laboratory of Biology and Genetic Resources of Rubber Tree, Ministry of Agriculture and Rural Affairs, Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou, China.
Tree Physiol ; 39(3): 391-403, 2019 03 01.
Article em En | MEDLINE | ID: mdl-30496555
Tapping panel dryness (TPD) causes a significant reduction in the latex yield of rubber tree (Hevea brasiliensis Muell. Arg.). It is reported that TPD is a typical programmed cell death (PCD) process. Although PCD plays a vital role in TPD occurrence, there is a lack of detailed and systematic study. Metacaspases are key regulators of diverse PCD in plants. Based on our previous result that HbMC1 was associated with TPD, we further elucidate the roles of HbMC1 on rubber tree TPD in this study. HbMC1 was up-regulated by TPD-inducing factors including wounding, ethephon and H2O2. Moreover, the expression level of HbMC1 was increased along with TPD severity in rubber tree, suggesting a positive correlation between HbMC1 expression and TPD severity. To investigate its biological function, HbMC1 was overexpressed in yeast (Saccharomyces cerevisiae) and tobacco (Nicotiana benthamiana). Transgenic yeast and tobacco overexpressing HbMC1 showed growth retardation compared with controls under H2O2-induced oxidative stress. In addition, overexpression of HbMC1 in yeast and tobacco reduced cell survival after high-concentration H2O2 treatment and resulted in enhanced H2O2-induced leaf cell death, respectively. A total of 11 proteins, rbcL, TM9SF2-like, COX3, ATP9, DRP, HbREF/Hevb1, MSSP2-like, SRC2, GATL8, CIPK14-like and STK, were identified and confirmed to interact with HbMC1 by yeast two-hybrid screening and co-transformation in yeast. The 11 proteins mentioned above are associated with many biological processes, including rubber biosynthesis, stress response, autophagy, carbohydrate metabolism, signal transduction, etc. Taken together, our results suggest that HbMC1-mediated PCD plays an important role in rubber tree TPD, and the identified HbMC1-interacting proteins provide valuable information for further understanding the molecular mechanism of HbMC1-mediated TPD in rubber tree.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Plantas / Morte Celular / Regulação da Expressão Gênica de Plantas / Caspases / Hevea / Látex Tipo de estudo: Prognostic_studies País/Região como assunto: America do sul / Brasil Idioma: En Revista: Tree Physiol Assunto da revista: BOTANICA / FISIOLOGIA Ano de publicação: 2019 Tipo de documento: Article País de afiliação: China País de publicação: Canadá
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Plantas / Morte Celular / Regulação da Expressão Gênica de Plantas / Caspases / Hevea / Látex Tipo de estudo: Prognostic_studies País/Região como assunto: America do sul / Brasil Idioma: En Revista: Tree Physiol Assunto da revista: BOTANICA / FISIOLOGIA Ano de publicação: 2019 Tipo de documento: Article País de afiliação: China País de publicação: Canadá