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Diversity and abundance of denitrifiers during cow manure composting.
Meng, Qingxin; Xu, Xiuhong; Zhang, Wenhao; Cheng, Lijun; Men, Mengqi; Xu, Benshu; Deng, Liting; Sun, Xuewei.
Afiliação
  • Meng Q; College of Resources and Environment, Northeast Agricultural University, Harbin 150030, China.
  • Xu X; College of Resources and Environment, Northeast Agricultural University, Harbin 150030, China. Electronic address: xuxiuhong@neau.edu.cn.
  • Zhang W; College of Resources and Environment, Northeast Agricultural University, Harbin 150030, China.
  • Cheng L; College of Resources and Environment, Northeast Agricultural University, Harbin 150030, China.
  • Men M; College of Resources and Environment, Northeast Agricultural University, Harbin 150030, China.
  • Xu B; College of Resources and Environment, Northeast Agricultural University, Harbin 150030, China.
  • Deng L; College of Resources and Environment, Northeast Agricultural University, Harbin 150030, China.
  • Sun X; College of Resources and Environment, Northeast Agricultural University, Harbin 150030, China.
Rev Argent Microbiol ; 51(3): 191-200, 2019.
Article em En | MEDLINE | ID: mdl-30467018
Diversity and abundance of the denitrifying genes nirK, nirS and nosZ were investigated in cow manure compost using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) and real-time quantitative PCR (qPCR), respectively. These three genes were detected in all the stages of the composting process. Phylogenetic analysis showed that the nirK gene was closely related to Rhizobiales, Burkholderiales, the nirS gene was closely related to Pseudomonadales and Burkholderiales, and the nosZ gene was closely related to Rhodospirillales, Rhizobiales, Pseudomonadales, and Alteromonadales. qPCR results showed that the abundance of these three genes (nirK, nirS and nosZ) reached the peak value in the late thermophilic stage of composting and abundance of the nirK gene was higher than that of the nosZ gene and the nirS gene. Redundancy analysis (RDA) showed that the diversity of the nirK and nirS genes was significantly correlated with ammonium (p<0.05), the diversity of the nosZ gene was significantly correlated with pH (p<0.05) and the abundance of the nirK nirS and nosZ genes was significantly correlated with temperature (p<0.05).
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Microbiologia do Solo / Compostagem / Desnitrificação / Genes Bacterianos Limite: Animals Idioma: En Revista: Rev Argent Microbiol Ano de publicação: 2019 Tipo de documento: Article País de afiliação: China País de publicação: Argentina
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Microbiologia do Solo / Compostagem / Desnitrificação / Genes Bacterianos Limite: Animals Idioma: En Revista: Rev Argent Microbiol Ano de publicação: 2019 Tipo de documento: Article País de afiliação: China País de publicação: Argentina