Diversity and abundance of denitrifiers during cow manure composting.
Rev Argent Microbiol
; 51(3): 191-200, 2019.
Article
em En
| MEDLINE
| ID: mdl-30467018
Diversity and abundance of the denitrifying genes nirK, nirS and nosZ were investigated in cow manure compost using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) and real-time quantitative PCR (qPCR), respectively. These three genes were detected in all the stages of the composting process. Phylogenetic analysis showed that the nirK gene was closely related to Rhizobiales, Burkholderiales, the nirS gene was closely related to Pseudomonadales and Burkholderiales, and the nosZ gene was closely related to Rhodospirillales, Rhizobiales, Pseudomonadales, and Alteromonadales. qPCR results showed that the abundance of these three genes (nirK, nirS and nosZ) reached the peak value in the late thermophilic stage of composting and abundance of the nirK gene was higher than that of the nosZ gene and the nirS gene. Redundancy analysis (RDA) showed that the diversity of the nirK and nirS genes was significantly correlated with ammonium (p<0.05), the diversity of the nosZ gene was significantly correlated with pH (p<0.05) and the abundance of the nirK nirS and nosZ genes was significantly correlated with temperature (p<0.05).
Palavras-chave
Compostaje; Composting; Denitrifying genes; Gen desnitrificador; PCR cuantitativa; Parámetro físico-químico; Physico-chemical parameters; Polymerase chain reaction-denaturing gradient gel electrophoresis; Quantitative PCR; Reacción en cadena de la polimerasa seguida de electroforesis en gel con gradiente de desnaturalización
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Microbiologia do Solo
/
Compostagem
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Desnitrificação
/
Genes Bacterianos
Limite:
Animals
Idioma:
En
Revista:
Rev Argent Microbiol
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
China
País de publicação:
Argentina