Fast and potent bactericidal membrane lytic activity of PaDBS1R1, a novel cationic antimicrobial peptide.
Biochim Biophys Acta Biomembr
; 1861(1): 178-190, 2019 01.
Article
em En
| MEDLINE
| ID: mdl-30463701
Antimicrobial peptides (AMPs) are promising candidates for the development of future antibiotics. In an attempt to increase the efficacy of therapeutic AMPs, computer-based design methods appear as a reliable strategy. In this study, we evaluated the antimicrobial efficiency and mechanism of action of a novel designed AMP named PaDBS1R1, previously designed by means of the Joker algorithm, using a fragment of the ribosomal protein L39E from the archaeon Pyrobaculum aerophilum as a template. PaDBS1R1 displayed low micromolar broad-spectrum antimicrobial activity against Gram-negative (MIC of 1.5⯵M) and Gram-positive (MIC of 3⯵M) bacteria, including carbapenem-resistant Klebsiella pneumoniae (MIC of 6.25⯵M) and methicillin-resistant Staphylococcus aureus (MIC of 12.5⯵M), without cytotoxicity towards HEK-293 cells. In addition, membrane permeabilization and depolarization assays, combined with time-kill studies and FEG-SEM imaging, indicated a fast membrane permeation and further leakage of intracellular content. Biophysical studies with lipid vesicles show a preference of PaDBS1R1 for Gram-negative bacteria-like membranes. We investigated the three-dimensional structure of PaDBS1R1 by CD and NMR analyses. Our results suggest that PaDBS1R1 adopts an amphipathic α-helix upon interacting with hydrophobic environments, after an initial electrostatic interaction with negative charges, suggesting a membrane lytic effect. This study reveals that PaDBS1R1 has potential application in antibiotic therapy.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Membrana Celular
/
Peptídeos Catiônicos Antimicrobianos
Limite:
Humans
Idioma:
En
Revista:
Biochim Biophys Acta Biomembr
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
Brasil
País de publicação:
Holanda