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Maternal immunization downregulates offspring TCD4 regulatory cells (Tregs) thymic maturation without implications for allergy inhibition.
de Oliveira, Marília Garcia; Lira, Aline Aparecida de Lima; Sgnotto, Fábio da Ressureição; Inoue, Amanda Harumi Sabô; Beltrame, Giovanna Rossi; da Silva, Débora; Menghini, Ricardo Palamar; Duarte, Alberto José da Silva; Victor, Jefferson Russo.
Afiliação
  • de Oliveira MG; Division of Dermatology, Laboratory of Medical Investigation LIM 56, Medical School, University of Sao Paulo, Sao Paulo, Brazil.
  • Lira AAL; Division of Dermatology, Laboratory of Medical Investigation LIM 56, Medical School, University of Sao Paulo, Sao Paulo, Brazil.
  • Sgnotto FDR; Division of Hematology, Medical School, University of Sao Paulo, Sao Paulo, Brazil.
  • Inoue AHS; Division of Dermatology, Laboratory of Medical Investigation LIM 56, Medical School, University of Sao Paulo, Sao Paulo, Brazil.
  • Beltrame GR; Division of Dermatology, Laboratory of Medical Investigation LIM 56, Medical School, University of Sao Paulo, Sao Paulo, Brazil.
  • da Silva D; Division of Environmental Health, FMU, Laureate International Universities, Sao Paulo, Brazil.
  • Menghini RP; Division of Environmental Health, FMU, Laureate International Universities, Sao Paulo, Brazil.
  • Duarte AJDS; Division of Dermatology, Laboratory of Medical Investigation LIM 56, Medical School, University of Sao Paulo, Sao Paulo, Brazil.
  • Victor JR; Division of Pathology, Medical School, University of Sao Paulo, Sao Paulo, Brazil.
Scand J Immunol ; 88(6): e12721, 2018 Dec.
Article em En | MEDLINE | ID: mdl-30403024
The regulation of offspring allergy development mediated by maternal immunization was evidenced by several groups, and this mechanism seems to involve the induction of regulatory T cells (Tregs) on offspring. Here, we aimed to evaluate whether the effect of maternal immunization on offspring Tregs occurs as a result of peripheral or central modulation. Briefly, C57BL/6 female mice were immunized with OVA in Alum or Alum alone and boosted with OVA in saline or saline only after 10 and 20 days. Non-immunized offspring serum, thymus and spleen were evaluated at 3 or 20 days old, and some groups of pups were submitted to neonatal OVA-immunization protocol for the subsequent evaluation of antibody production and allergic response. Our experimental protocol could be validated because maternal OVA-immunization inhibited offspring allergic response as evidenced by the suppression of offspring IgE production and allergic lung inflammation. Interestingly, maternal immunization reduced the frequency of offspring thymic Tregs with an opposite effect on spleen Tregs. Furthermore, after neonatal immunization, the frequency of lung-infiltrated Tregs was also augmented on offspring from immunized mothers. In conclusion, maternal OVA-immunization can inhibit the thymic maturation of offspring Tregs without implications on peripheral Tregs induction and allergy inhibition.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pneumonia / Baço / Timo / Linfócitos T Reguladores / Hipersensibilidade Limite: Animals / Pregnancy Idioma: En Revista: Scand J Immunol Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pneumonia / Baço / Timo / Linfócitos T Reguladores / Hipersensibilidade Limite: Animals / Pregnancy Idioma: En Revista: Scand J Immunol Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido