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13-loci STR multiplex system for Brazilian seized samples of marijuana: individualization and origin differentiation.
Fett, Mauro Sander; Mariot, Roberta Fogliatto; Avila, Eduardo; Alho, Clarice Sampaio; Stefenon, Valdir Marcos; de Oliveira Camargo, Flávio Anastácio.
Afiliação
  • Fett MS; Soil Science Department, Agronomy Faculty, Federal University of Rio Grande do Sul (UFRGS), Avenida Bento Gonçalves, 7712, Porto Alegre, Rio Grande do Sul, CEP 91540-000, Brazil.
  • Mariot RF; Setor Técnico-Científico, Superintendência Regional do Departamento de Polícia Federal no Rio Grande do Sul, Avenida Ipiranga, 1365, Bairro Azenha, Porto Alegre, Rio Grande do Sul, CEP 90160-093, Brazil.
  • Avila E; Soil Science Department, Agronomy Faculty, Federal University of Rio Grande do Sul (UFRGS), Avenida Bento Gonçalves, 7712, Porto Alegre, Rio Grande do Sul, CEP 91540-000, Brazil. rfmariot@gmail.com.
  • Alho CS; Setor Técnico-Científico, Superintendência Regional do Departamento de Polícia Federal no Rio Grande do Sul, Avenida Ipiranga, 1365, Bairro Azenha, Porto Alegre, Rio Grande do Sul, CEP 90160-093, Brazil.
  • Stefenon VM; Bioscience Faculty, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Avenida Ipiranga, 6681, Bairro Partenon, Porto Alegre, Rio Grande do Sul, CEP 90619-900, Brazil.
  • de Oliveira Camargo FA; Bioscience Faculty, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Avenida Ipiranga, 6681, Bairro Partenon, Porto Alegre, Rio Grande do Sul, CEP 90619-900, Brazil.
Int J Legal Med ; 133(2): 373-384, 2019 Mar.
Article em En | MEDLINE | ID: mdl-30324327
It is known that Cannabis in Brazil could either originate from Paraguay or be cultivated in Brazil. While consumer markets in the North and Northeast regions are maintained by national production, the rest of the country is supplied with Cannabis from Paraguay. However, the Brazilian Federal Police (BFP) has exponentially increased the seizure number of Cannabis seeds sent by mail. For this reason, the aim of the study was to assess the 13-loci short tandem repeat (STR) multiplex system proposed by Houston et al. (2015) to evaluate the power of such markers in individualization and origin differentiation of Cannabis sativa samples seized in Brazil by the BFP. To do so, 72 Cannabis samples seized in Brazil by BFP were analyzed. The principal coordinate analysis (PCoA) and probability identity (PI) analysis were computed. Additionally, the Cannabis samples' genotypes were subjected to comparison by Kruskal-Wallis H, followed by a multiple discriminant analysis (MDA). All samples analyzed revealed a distinct genetic profile. PCoA clearly discriminated the seizure sets based on their geographic origin. A combination of seven loci was enough to differentiate samples' genotypes, and the PI for a random sample is approximately one in 50 billion. The Cannabis samples were 100% correct as classified by Kruskal-Wallis H, followed by an MDA. The results of this study demonstrate that the 13-loci STR multiplex system successfully achieved the aim of sample individualization and origin differentiation and suggest that it could be a useful tool to help BFP intelligence in tracing back-trade routes.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Cannabis / Repetições de Microssatélites / Tráfico de Drogas / Genótipo Limite: Humans País/Região como assunto: America do sul / Brasil Idioma: En Revista: Int J Legal Med Assunto da revista: JURISPRUDENCIA Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Brasil País de publicação: Alemanha
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Cannabis / Repetições de Microssatélites / Tráfico de Drogas / Genótipo Limite: Humans País/Região como assunto: America do sul / Brasil Idioma: En Revista: Int J Legal Med Assunto da revista: JURISPRUDENCIA Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Brasil País de publicação: Alemanha