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Chemically modified alginate bead matrix for efficient adsorptive recovery of trypsin from fresh bovine pancreas.
Aravena, Pilar; Brassesco, Maria Emilia; Bosio, Barbara; Picó, Guillermo; Woitovich Valetti, Nadia.
Afiliação
  • Aravena P; Faculty of Biochemical and Pharmaceutical Sciences, Inst. of Biotechnological and Chemistry Processes, CONICET, National University of Rosario, Rosario, Argentina.
  • Brassesco ME; Faculty of Biochemical and Pharmaceutical Sciences, Inst. of Biotechnological and Chemistry Processes, CONICET, National University of Rosario, Rosario, Argentina.
  • Bosio B; Faculty of Biochemical and Pharmaceutical Sciences, Inst. of Biotechnological and Chemistry Processes, CONICET, National University of Rosario, Rosario, Argentina.
  • Picó G; Faculty of Biochemical and Pharmaceutical Sciences, Inst. of Biotechnological and Chemistry Processes, CONICET, National University of Rosario, Rosario, Argentina.
  • Woitovich Valetti N; Faculty of Biochemical and Pharmaceutical Sciences, Inst. of Biotechnological and Chemistry Processes, CONICET, National University of Rosario, Rosario, Argentina.
Biotechnol Prog ; 34(5): 1269-1277, 2018 09.
Article em En | MEDLINE | ID: mdl-30281952
The adsorption of commercial trypsin (Try) onto epichlorohydrin cross-linked alginate-guar gum matrix has been studied at equilibrium in batch and in fixed bed column. Experiments were conducted to study the effect of ionic strength, temperature and to obtain a thermodynamic characterization of the adsorption process. The resulting adsorption isotherm fitted the Hill equation. Experimental breakthrough curve profiles were compared with the theoretical breakthrough profiles obtained from the mathematical model, bed depth service time. At pH 5.0, 1.0 g hydrated matrix adsorbed 480.0 milligram of Try per gram of dried bed. The desorption process showed 80% of Try recovery in 50 mM phosphate buffer, pH 7.00-500 mM NaCl-20% propylene glycol. The obtained results were applied to an adsorption/washing/desorption process with fresh pancreas homogenate yielded 20% of recovery and 5.7 purification factor of Try. The matrix remained functional until the fifth cycle of repeated batch enzyme adsorption. © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 2018.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pâncreas / Tripsina / Alginatos Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Biotechnol Prog Assunto da revista: BIOTECNOLOGIA Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Argentina País de publicação: Estados Unidos
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pâncreas / Tripsina / Alginatos Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Biotechnol Prog Assunto da revista: BIOTECNOLOGIA Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Argentina País de publicação: Estados Unidos