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A screening test based on hematological and histological biomarkers to evaluate the environmental impacts in tambaqui (Colossoma macropomum) from a protected area in Maranhão, Brazilian Amazon.
Pinheiro-Sousa, Débora Batista; Torres Junior, Audalio Rebelo; Silva, Dilson; Santos, Ricardo Luvizotto; Fortes Carvalho Neta, Raimunda Nonata.
Afiliação
  • Pinheiro-Sousa DB; PhD Student in Biodiversity and Biotechnology of the Legal Amazon (Rede Bionorte, Brazil), Department of Environmental Engineering, Federal University of Maranhão (UFMA), Balsas, Maranhão, Brazil. Electronic address: debora.batista@ufma.br.
  • Torres Junior AR; Marine Sciences Institute, Federal University of Maranhão (UFMA), São Luis, Maranhão, Brazil.
  • Silva D; Applied Mathematics, State University of Rio de Janeiro (UERJ), Rio de Janeiro, Brazil.
  • Santos RL; Department of Oceanography and Limnology, Federal University of Maranhão (UFMA), São Luis, Maranhão, Brazil.
  • Fortes Carvalho Neta RN; Department of Chemistry and Biology, State University of Maranhão (UEMA), Campus Paulo VI, São Luis, Maranhão, Brazil.
Chemosphere ; 214: 445-451, 2019 Jan.
Article em En | MEDLINE | ID: mdl-30273878
Nowadays biomonitoring programs can benefit with mathematical models able to correlate biomarkers to monitor water pollution. The aim of this study was to develop a screening test based on hematological parameters and histological lesions in tambaqui (Colossoma macropomum), to allow the assessment of environmental impacts on fish inhabiting a protected area in Maranhão inside of Brazilian Amazon. Samples collected during three years (2012, 2013 and 2014) were grouped by season (dry and rainy) Water samples were also collected for physical chemistry analysis. Blood samples were stained with Acridine Orange to detect micronuclei and erythrocyte abnormalities. Gill tissues were stained with hematoxylin and counterstained with alcoholic eosin, and histopathological lesions were scored on a scale of 1-3, being 1 = minimal pathological importance, 2 = moderate pathological importance and 3 = marked pathological importance. A screening test for evaluating environmental impact was developed by fitting the measured data (necrosis, erythrocyte abnormalities, number of micronuclei) from tambaqui. A three-dimensional surface was fit to the empirical data. Our proposed model predicted the probability of necrosis (observed in euthanized animals) based on the numbers of micronuclei and abnormal erythrocytes (observed in blood samples from live animals) (correlation coefficient R = 0.89). The methodology could be applied for predicting contamination histories (chronic pollution that induces branchial lesions) in rivers using the micronucleus and erythrocyte abnormalities of the fishes (with a simple blood sample).
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Biomarcadores / Monitoramento Ambiental / Micronúcleos com Defeito Cromossômico / Exposição Ambiental / Eritrócitos / Peixes / Necrose Tipo de estudo: Diagnostic_studies / Prognostic_studies / Screening_studies Limite: Animals País/Região como assunto: America do sul / Brasil Idioma: En Revista: Chemosphere Ano de publicação: 2019 Tipo de documento: Article País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Biomarcadores / Monitoramento Ambiental / Micronúcleos com Defeito Cromossômico / Exposição Ambiental / Eritrócitos / Peixes / Necrose Tipo de estudo: Diagnostic_studies / Prognostic_studies / Screening_studies Limite: Animals País/Região como assunto: America do sul / Brasil Idioma: En Revista: Chemosphere Ano de publicação: 2019 Tipo de documento: Article País de publicação: Reino Unido