De novo domestication of wild tomato using genome editing.

Zsögön, Agustin; Cermák, Tomás; Naves, Emmanuel Rezende; Notini, Marcela Morato; Edel, Kai H; Weinl, Stefan; Freschi, Luciano; Voytas, Daniel F; Kudla, Jörg; Peres, Lázaro Eustáquio Pereira

Zsögön, Agustin; Cermák, Tomás; Naves, Emmanuel Rezende; Notini, Marcela Morato; Edel, Kai H; Weinl, Stefan; Freschi, Luciano; Voytas, Daniel F; Kudla, Jörg; Peres, Lázaro Eustáquio Pereira.

Afiliação

Zsögön A; Departamento de Biologia Vegetal, Universidade Federal de Viçosa, Viçosa, Brazil.
Cermák T; Department of Genetics, Cell Biology and Development, Center for Genome Engineering, University of Minnesota, Minneapolis, Minnesota, USA.
Naves ER; Departamento de Biologia Vegetal, Universidade Federal de Viçosa, Viçosa, Brazil.
Notini MM; Departamento de Ciências Biológicas, Escola Superior de Agricultura "Luiz de Queiroz," Universidade de São Paulo, Piracicaba, Brazil.
Edel KH; Institut für Biologie und Biotechnologie der Pflanzen, Universität Münster, Münster, Germany.
Weinl S; Institut für Biologie und Biotechnologie der Pflanzen, Universität Münster, Münster, Germany.
Freschi L; Instituto de Biociências, Universidade de São Paulo, São Paulo, Brazil.
Voytas DF; Department of Genetics, Cell Biology and Development, Center for Genome Engineering, University of Minnesota, Minneapolis, Minnesota, USA.
Kudla J; Institut für Biologie und Biotechnologie der Pflanzen, Universität Münster, Münster, Germany.
Peres LEP; Departamento de Ciências Biológicas, Escola Superior de Agricultura "Luiz de Queiroz," Universidade de São Paulo, Piracicaba, Brazil.

Nat Biotechnol ; 2018 Oct 01.

Article em En | MEDLINE | ID: mdl-30272678

RESUMO

Breeding of crops over millennia for yield and productivity has led to reduced genetic diversity. As a result, beneficial traits of wild species, such as disease resistance and stress tolerance, have been lost. We devised a CRISPR-Cas9 genome engineering strategy to combine agronomically desirable traits with useful traits present in wild lines. We report that editing of six loci that are important for yield and productivity in present-day tomato crop lines enabled de novo domestication of wild Solanum pimpinellifolium. Engineered S. pimpinellifolium morphology was altered, together with the size, number and nutritional value of the fruits. Compared with the wild parent, our engineered lines have a threefold increase in fruit size and a tenfold increase in fruit number. Notably, fruit lycopene accumulation is improved by 500% compared with the widely cultivated S. lycopersicum. Our results pave the way for molecular breeding programs to exploit the genetic diversity present in wild plants.

Texto completo

Adicionar na Minha BVS

Imprimir

XML

PubMed Links

Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Nat Biotechnol Assunto da revista: BIOTECNOLOGIA Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos

Texto completo

Adicionar na Minha BVS

Imprimir

XML

PubMed Links

Buscar no Google