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Deletion of BmoR affects the expression of genes related to thiol/disulfide balance in Bacteroides fragilis.
Teixeira, Felipe L; Pauer, Heidi; Costa, Scarlathe B; Smith, C Jeffrey; Domingues, Regina M C P; Rocha, Edson R; Lobo, Leandro A.
Afiliação
  • Teixeira FL; Departamento de Microbiologia Médica, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil. teixeirafl@micro.ufrj.br.
  • Pauer H; Department of Microbiology and Immunology, Brody School of Medicine, East Carolina University, Greenville, NC, USA. teixeirafl@micro.ufrj.br.
  • Costa SB; Departamento de Microbiologia Médica, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
  • Smith CJ; Department of Microbiology and Immunology, Brody School of Medicine, East Carolina University, Greenville, NC, USA.
  • Domingues RMCP; Departamento de Microbiologia Médica, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
  • Rocha ER; Department of Microbiology and Immunology, Brody School of Medicine, East Carolina University, Greenville, NC, USA.
  • Lobo LA; Departamento de Microbiologia Médica, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
Sci Rep ; 8(1): 14405, 2018 09 26.
Article em En | MEDLINE | ID: mdl-30258073
Bacteroides fragilis, an opportunistic pathogen and commensal bacterium in the gut, is one the most aerotolerant species among strict anaerobes. However, the mechanisms that control gene regulation in response to oxidative stress are not completely understood. In this study, we show that the MarR type regulator, BmoR, regulates the expression of genes involved in the homeostasis of intracellular redox state. Transcriptome analysis showed that absence of BmoR leads to altered expression in total of 167 genes. Sixteen of these genes had a 2-fold or greater change in their expression. Most of these genes are related to LPS biosynthesis and carbohydrates metabolism, but there was a significant increase in the expression of genes related to the redox balance inside the cell. A pyridine nucleotide-disulfide oxidoreductase located directly upstream of bmoR was shown to be repressed by direct binding of BmoR to the promoter region. The expression of two other genes, coding for a thiosulphate:quinone-oxidoreductase and a thioredoxin, are indirectly affected by bmoR mutation during oxygen exposure. Phenotypic assays showed that BmoR is important to maintain the thiol/disulfide balance in the cell, confirming its relevance to B. fragilis response to oxidative stress.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas Repressoras / Compostos de Sulfidrila / Bacteroides fragilis / Regulação Bacteriana da Expressão Gênica / Deleção de Genes / Estresse Oxidativo / Dissulfetos Idioma: En Revista: Sci Rep Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas Repressoras / Compostos de Sulfidrila / Bacteroides fragilis / Regulação Bacteriana da Expressão Gênica / Deleção de Genes / Estresse Oxidativo / Dissulfetos Idioma: En Revista: Sci Rep Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido