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Amblyomin-X, a recombinant Kunitz-type inhibitor, regulates cell adhesion and migration of human tumor cells.
Schmidt, Mariana Costa Braga; Morais, Katia L P; Almeida, Maíra Estanislau Soares de; Iqbal, Asif; Goldfeder, Mauricio Barbugiani; Chudzinski-Tavassi, Ana Marisa.
Afiliação
  • Schmidt MCB; Laboratory of Molecular Biology, Butantan Institute , São Paulo, SP, Brazil.
  • Morais KLP; Departament of Biochemistry, Federal University of São Paulo , São Paulo, SP, Brazil.
  • Almeida MES; Laboratory of Molecular Biology, Butantan Institute , São Paulo, SP, Brazil.
  • Iqbal A; Departament of Biochemistry, Federal University of São Paulo , São Paulo, SP, Brazil.
  • Goldfeder MB; Centre of Excellence in New Target Discovery, Butantan Institute , São Paulo, SP, Brazil.
  • Chudzinski-Tavassi AM; Laboratory of Molecular Biology, Butantan Institute , São Paulo, SP, Brazil.
Cell Adh Migr ; 14(1): 129-138, 2020 12.
Article em En | MEDLINE | ID: mdl-30238848
In a tumor microenvironment, endothelial cell migration and angiogenesis allow cancer to spread to other organs causing metastasis.  Indeed, a number of molecules that are involved in cytoskeleton re-organization and intracellular signaling have been investigated for their effects on tumor cell growth and metastasis. Alongside that, Amblyomin-X, a recombinant Kunitz-type protein, has been shown to reduce metastasis and tumor growth in in vivo experiments. In the present report, we provide a mechanistic insight to these antitumor effects, this is,  Amblyomin-X modulates Rho-GTPases and uPAR signaling, and reduces the release of MMPs, leading to disruption of the actin cytoskeleton and decreased cell migration of tumor cell lines. Altogether, our data support a role for Amblyomin-X as a novel potential antitumor drug. ABBREVIATIONS: Amb-X: Amblyomin-X; ECGF: endotelial cell growth factor; ECM: extracellular matrix; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; HUVEC: human umbilical vein endothelial cell; LRP1: low-density lipoprotein receptor-related protein; MMP: matrix metalloproteinase; HPI-4: hedgehog pathway inhibitor 4; PAI-1: plasminogen activator inhibitor 1; PMA: phorbol 12-myristate-13-acetate; TFPI: tissue factor pathway inhibitor; uPA: urokinase plasminogen activator; uPAR: uPA receptor.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas e Peptídeos Salivares / Proteínas Recombinantes / Movimento Celular / Aprotinina / Proteínas de Artrópodes Limite: Humans Idioma: En Revista: Cell Adh Migr Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos
Texto completo
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XML
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas e Peptídeos Salivares / Proteínas Recombinantes / Movimento Celular / Aprotinina / Proteínas de Artrópodes Limite: Humans Idioma: En Revista: Cell Adh Migr Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos