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Fish Oil Supplementation Reduces Inflammation but Does Not Restore Renal Function and Klotho Expression in an Adenine-Induced CKD Model.
Henao Agudelo, Juan S; Baia, Leandro C; Ormanji, Milene S; Santos, Amandda R P; Machado, Juliana R; Saraiva Câmara, Niels O; Navis, Gerjan J; de Borst, Martin H; Heilberg, Ita P.
Afiliação
  • Henao Agudelo JS; Division of Nephrology, Federal University of São Paulo (UNIFESP), Rua Botucatu 740, 04023-900 São Paulo, Brazil. juanelmono17@hotmail.com.
  • Baia LC; Division of Nephrology, Federal University of São Paulo (UNIFESP), Rua Botucatu 740, 04023-900 São Paulo, Brazil. leandronut@yahoo.com.br.
  • Ormanji MS; Division of Nephrology, University of Groningen, University Medical Centre Groningen (UMCG), P.O. Box 30.001, 9700 RB Groningen, The Netherlands. leandronut@yahoo.com.br.
  • Santos ARP; Division of Nephrology, Federal University of São Paulo (UNIFESP), Rua Botucatu 740, 04023-900 São Paulo, Brazil. milene.ormanji@gmail.com.
  • Machado JR; Division of Nephrology, Federal University of São Paulo (UNIFESP), Rua Botucatu 740, 04023-900 São Paulo, Brazil. amandda.rpds@gmail.com.
  • Saraiva Câmara NO; Tropical Medicine & Public Health, Federal University of Goiás (UFG), Rua 235 s/n-University Sector, 74605-050 Goiânia, Brazil. juliana.patologiageral@gmail.com.
  • Navis GJ; Division of Nephrology, Federal University of São Paulo (UNIFESP), Rua Botucatu 740, 04023-900 São Paulo, Brazil. niels@icb.usp.br.
  • de Borst MH; Department of Immunology, Institute of Biomedical Sciences, University of São Paulo (USP), Av. Prof. Lineu Prestes 1730, ICB IV, Sala 238, 05508-000 São Paulo, Brazil. niels@icb.usp.br.
  • Heilberg IP; Division of Nephrology, University of Groningen, University Medical Centre Groningen (UMCG), P.O. Box 30.001, 9700 RB Groningen, The Netherlands. g.j.navis@umcg.nl.
Nutrients ; 10(9)2018 Sep 11.
Article em En | MEDLINE | ID: mdl-30208590
BACKGROUND: Chronic kidney disease and inflammation promote loss of Klotho expression. Given the well-established anti-inflammatory effects of omega-3 fatty acids, we aimed to investigate the effect of fish oil supplementation in a model of CKD. METHODS: Male C57BL/6 mice received supplementation with an adenine-enriched diet (AD, n = 5) or standard diet (CTL, n = 5) for 10 days. Two other experimental groups were kept under the adenine diet for 10 days. Following adenine withdrawal on the 11th day, the animals returned to a standard diet supplemented with fish oil (Post AD-Fish oil, n = 9) or not (Post AD-CTL, n = 9) for an additional period of 7 days. RESULTS: Adenine mice exhibited significantly higher mean serum urea, creatinine, and renal expression of the pro-inflammatory markers Interleukin-6 (IL-6), C-X-C motif chemokine 10 (CXCL10), and Interleukin-1ß (IL-1ß), in addition to prominent renal fibrosis and reduced renal Klotho gene expression compared to the control. Post AD-Fish oil animals demonstrated a significant reduction of IL-6, C-X-C motif chemokine 9 (CXCL9), and IL-1ß compared to Post AD-CTL animals. However, serum creatinine, renal fibrosis, and Klotho were not significantly different in the fish oil-treated group. Furthermore, renal histomorphological changes such as tubular dilatation and interstitial infiltration persisted despite treatment. CONCLUSIONS: Fish oil supplementation reduced renal pro-inflammatory markers but was not able to restore renal function nor Klotho expression in an adenine-induced CKD model.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Óleos de Peixe / Adenina / Mediadores da Inflamação / Suplementos Nutricionais / Insuficiência Renal Crônica / Rim / Proteínas de Membrana / Nefrite Limite: Animals Idioma: En Revista: Nutrients Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Brasil País de publicação: Suíça
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Óleos de Peixe / Adenina / Mediadores da Inflamação / Suplementos Nutricionais / Insuficiência Renal Crônica / Rim / Proteínas de Membrana / Nefrite Limite: Animals Idioma: En Revista: Nutrients Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Brasil País de publicação: Suíça