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Comparative proteomics of soluble factors secreted by human breast adipose tissue from tumor and normal breast.
Fletcher, Sabrina Johanna; Hapon, María Belén; Callegari, Eduardo A; Crosbie, María Luján; Santiso, Natalia; Ursino, Anabela; Amato, Alicia Rita; Gutiérrez, Alberto; Sacca, Paula Alejandra; Dreszman, Rubén; Pérez, Adriana; Carón, Rubén Walter; Calvo, Juan Carlos; Pistone-Creydt, Virginia.
Afiliação
  • Fletcher SJ; Laboratorio de Química de Proteoglicanos y Matriz Extracelular, Instituto de Biología y Medicina Experimental (IBYME), Buenos Aires, Argentina.
  • Hapon MB; Laboratorio de Reproducción y Lactancia, Instituto de Medicina y Biología Experimental de Cuyo (IMBECU), Centro Científico y Tecnológico Mendoza, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Mendoza, Argentina.
  • Callegari EA; University of South Dakota, Sanford School of Medicine, Vermillion, South Dakota, USA.
  • Crosbie ML; Sección de Patología Mamaria, Servicio de Ginecología, Complejo Médico Policial "Churruca-Visca", Buenos Aires, Argentina.
  • Santiso N; Sección de Patología Mamaria, Servicio de Ginecología, Complejo Médico Policial "Churruca-Visca", Buenos Aires, Argentina.
  • Ursino A; Sección de Patología Mamaria, Servicio de Ginecología, Complejo Médico Policial "Churruca-Visca", Buenos Aires, Argentina.
  • Amato AR; Sección de Patología Mamaria, Servicio de Ginecología, Complejo Médico Policial "Churruca-Visca", Buenos Aires, Argentina.
  • Gutiérrez A; Sección de Patología Mamaria, Servicio de Ginecología, Complejo Médico Policial "Churruca-Visca", Buenos Aires, Argentina.
  • Sacca PA; Laboratorio de Química de Proteoglicanos y Matriz Extracelular, Instituto de Biología y Medicina Experimental (IBYME), Buenos Aires, Argentina.
  • Dreszman R; Clínica de Microcirugía, Buenos Aires, Argentina.
  • Pérez A; Departamento de Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina.
  • Carón RW; Laboratorio de Hormonas y Biología del Cáncer, Instituto de Medicina y Biología Experimental de Cuyo (IMBECU), Centro Científico y Tecnológico Mendoza, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Mendoza, Argentina.
  • Calvo JC; Laboratorio de Química de Proteoglicanos y Matriz Extracelular, Instituto de Biología y Medicina Experimental (IBYME), Buenos Aires, Argentina.
  • Pistone-Creydt V; Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina.
Oncotarget ; 9(57): 31007-31017, 2018 Jul 24.
Article em En | MEDLINE | ID: mdl-30123423
Tumor progression depends on the tumor-stroma interaction. In the breast, adipose tissue is the predominant stromal type. We have previously demonstrated that conditioned media (CMs) from explants of human adipose tissue of tumor breasts (hATT) increase proliferation and migration of breast cancer epithelial cells when compared to human adipose tissue from normal breasts (hATN). In this work, we aim to identify specific proteins and molecular/biological pathways associated with the secretion profile of hATT and hATN explants. hATT-CMs and hATN-CMs were separated by SDS-PAGE and analyzed by means of two-dimensional nano-liquid chromatography-mass spectrometry. The data was analyzed using ProteoIQ and FunRich software. In addition, 42 cytokines from hATT-CMs and hATN-CMs were assayed by a protein antibody assay. Compared to hATN-CMs, hATT-CMs showed greater protein diversity. We found that hATT-CMs presented a greater amount of proteins related to complement system activity, metabolism and immune system, as well as proteins involved in a variety of biological processes such as signal transduction and cell communication. Specifically, apolipoprotein AI and AII, complement component 3, and vimentin and desmin were significantly increased in hATT-CMs versus hATN-CMs. Moreover, a multivariate discriminant analysis of the cytokines detected by the array showed that IL-6, MCP-2 and GRO cytokines were sufficient and necessary to differentiate hATT-CMs from hATN-CMs. This analysis also showed that the levels of these three cytokines, taken together, correlated with stage and histological grade of the tumor in the hATT-CMs group, and with body mass index in the hATN-CMs group.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Prognostic_studies Idioma: En Revista: Oncotarget Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Argentina País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Prognostic_studies Idioma: En Revista: Oncotarget Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Argentina País de publicação: Estados Unidos