The usefulness of short-term high-fat/high salt diet as a model of metabolic syndrome in mice.
Life Sci
; 209: 341-348, 2018 Sep 15.
Article
em En
| MEDLINE
| ID: mdl-30118771
Diabetic cardiomyopathy (DC) describes diabetes-associated changes in the structure and function of myocardium that are not directly linked to other factors such as hypertension. Currently there are some models of DC; however, they take a large time period to mimic key features. In the present study, we investigated the effects of a short-term high-fat/high salt diet (HFHS) treatment on myocardial function and structure, and vascular reactivity in C57BL/6 male mice. After 14â¯weeks HFHS induced hypertension (MAPâ¯=â¯144.95⯱â¯16.13 vs 92.90⯱â¯18.95â¯mmâ¯Hg), low glucose tolerance (AUCâ¯=â¯1049.01⯱â¯74.79 vs 710.50⯱â¯52.57â¯a.u.), decreased insulin sensitivity (AUCâ¯=â¯429.83⯱â¯35.22 vs 313.67⯱â¯19.55â¯a.u.) and increased adiposity (epididymal fat weight 0.96⯱â¯0.10 vs 0.59⯱â¯0.06 OW/BWâ¯×â¯102), aspects present in metabolic syndrome. Cardiac evaluation showed diastolic dysfunction (E/A ratioâ¯=â¯1.20 vs 1.90â¯u.a.) and cardiomyocyte hypertrophy (cardiomyocyte areaâ¯=â¯502.82⯱â¯31.46 vs 385.58⯱â¯22.11⯵m2). Lastly, vascular reactivity was impaired with higher contractile response (136.10⯱â¯3.49 vs 120.37⯱â¯5.43%) and lower response to endothelium-dependent vasorelaxation (74.01⯱â¯4.35 vs 104.84⯱â¯3.57%). In addition, the diet was able to induce an inward coronary remodeling (vascular total area: SCNS 6185⯱â¯800.6 vs HFHS 4085⯱â¯213.7⯵m2). Therefore, we conclude that HFHS short-term treatment was able to induce metabolic syndrome-like state, cardiomyopathy and vascular injury working as an important tool to study cardiometabolic diseases.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cloreto de Sódio na Dieta
/
Síndrome Metabólica
/
Dieta Hiperlipídica
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Cardiomiopatias
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Life Sci
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
Brasil
País de publicação:
Holanda