Serum antiphospholipid antibody levels as biomarkers for diagnosis of pulmonary tuberculosis patients.

Takenami, I; de Oliveira, C C; Petrilli, J D; Machado, A; Riley, L W; Arruda, S

Takenami, I; de Oliveira, C C; Petrilli, J D; Machado, A; Riley, L W; Arruda, S.

Afiliação

Takenami I; Laboratório Avançado de Saúde Pública, Instituto Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, Bahia.
de Oliveira CC; Laboratório Avançado de Saúde Pública, Instituto Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, Bahia.
Petrilli JD; Laboratório Avançado de Saúde Pública, Instituto Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, Bahia.
Machado A; Departamiento de Ciências da Vida, Universidade Estadual de Bahia, Salvador, Bahia, Hospital Especializado Octávio Mangabeira, Secretaria da Saúde do Estado da Bahia, Salvador, Bahia, Brazil.
Riley LW; Division of Infectious Diseases and Vaccinology, School of Public Health, University of California, Berkeley, California, USA.
Arruda S; Laboratório Avançado de Saúde Pública, Instituto Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, Bahia, Departamiento de Ciências da Vida, Universidade Estadual de Bahia, Salvador, Bahia.

Int J Tuberc Lung Dis ; 22(9): 1063-1070, 2018 09 01.

Article em En | MEDLINE | ID: mdl-30092873

RESUMO

SETTING: Salvador, Bahia, Brazil. OBJECTIVE: To evaluate the immunoglobulin (Ig)M and total IgG antibody response to cardiolipin (CL), phosphatidylcholine (PTC), phosphatidylethanolamine (PE), phosphatidylinositol (PI) and sulfatide (SL-I) as biosignatures that can be used to diagnose pulmonary tuberculosis (TB) and its applicability for monitoring the efficacy of anti-tuberculosis treatment. DESIGN: Serum samples from 37 adult pulmonary TB patients and 48 controls (16 healthy household contacts, 19 household contacts with latent tuberculous infection [LTBI] and 13 non-TB patients with lung disease) were screened using enzyme-linked immunosorbent assays (ELISAs) for IgM and total IgG against phospholipids. RESULTS: Levels of IgM response to CL, PE and PI, and IgG response to CL, PE, PI and PTC were significantly higher in TB patients than in control groups. Anti-CL IgG had the best performance characteristics, with a sensitivity and specificity of respectively 86.5% and 87.2%. This IgG anti-CL ELISA test detected 86.5% (32/37) of the TB patients, whereas the number detected using sputum smear was only 65.9% (24/37). After anti-tuberculosis treatment, the median value for all anti-phospholipid antibodies decreased significantly compared with baseline values (P < 0.05). CONCLUSION: Our results suggest that the total IgG anti-CL level could be useful to complement conventional bacteriological tests for the rapid diagnosis of adult pulmonary TB.

Assuntos

Anticorpos Antifosfolipídeos/sangue; Mycobacterium tuberculosis/isolamento & purificação; Escarro/microbiologia; Tuberculose Pulmonar/diagnóstico; Adulto; Biomarcadores/sangue; Brasil; Estudos Transversais; Ensaio de Imunoadsorção Enzimática; Humanos; Imunoglobulina G/sangue; Imunoglobulina M/sangue; Sensibilidade e Especificidade; Tuberculose Pulmonar/sangue

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Escarro / Tuberculose Pulmonar / Anticorpos Antifosfolipídeos / Mycobacterium tuberculosis Tipo de estudo: Diagnostic_studies / Observational_studies / Prevalence_studies / Risk_factors_studies Limite: Adult / Humans País/Região como assunto: America do sul / Brasil Idioma: En Revista: Int J Tuberc Lung Dis Ano de publicação: 2018 Tipo de documento: Article País de publicação: França

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