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Comparative analysis of real-time PCR assays in the detection of canine visceral leishmaniasis.
Nunes, Juliana Barbosa; Coura-Vital, Wendel; Colombo, Fabio Antônio; Baêta, Frederico José Moreira; Pinheiro, Aimara Costa; Roatt, Bruno Mendes; Reis, Levi Eduardo Soares; Reis, Alexandre Barbosa; Marques, Marcos José.
Afiliação
  • Nunes JB; College of Medicine, Department of Pathology, University of São Paulo, São Paulo, São Paulo, Brazil.
  • Coura-Vital W; Nucleus of Research in Biological Sciences (NUPEB), Federal University of Ouro Preto, Ouro Preto, Minas Gerais, Brazil.
  • Colombo FA; Pharmaceutical Sciences Faculty, Laboratory of Clinical Parasitology, Universidade Federal de Alfenas, Alfenas, Minas Gerais, Brazil.
  • Baêta FJM; Biomedical Sciences Institute, Universidade Federal de Alfenas - UNIFAL-MG, Gabriel Monteiro da Silva Street, 700, Alfenas, Minas Gerais, 37130-001, Brazil.
  • Pinheiro AC; Municipal Health Secretariat, Zoonosis Control Center, Governador Valadares, Minas Gerais, Brazil.
  • Roatt BM; Nucleus of Research in Biological Sciences (NUPEB), Federal University of Ouro Preto, Ouro Preto, Minas Gerais, Brazil.
  • Reis LES; Nucleus of Research in Biological Sciences (NUPEB), Federal University of Ouro Preto, Ouro Preto, Minas Gerais, Brazil.
  • Reis AB; Nucleus of Research in Biological Sciences (NUPEB), Federal University of Ouro Preto, Ouro Preto, Minas Gerais, Brazil.
  • Marques MJ; Biomedical Sciences Institute, Universidade Federal de Alfenas - UNIFAL-MG, Gabriel Monteiro da Silva Street, 700, Alfenas, Minas Gerais, 37130-001, Brazil. marques@unifal-mg.edu.br.
Parasitol Res ; 117(10): 3341-3346, 2018 Oct.
Article em En | MEDLINE | ID: mdl-30088073
Dogs are important hosts and reservoirs of leishmaniasis, a disease caused by protozoan parasites from the genus Leishmania, affecting ~12 million people worldwide. The detection of visceral leishmaniasis (VL) in dogs by real-time PCR (qPCR) may improve on diagnosis, but the different qPCR methods available for Leishmania DNA detection have not been established as routine in diagnostic tools and/or epidemiologic studies for canine VL. Here, we compared three qPCR assays (DNApol, Linj31, and LDON) in the detection of VL by Leishmania infantum in spleen (n = 48; 7), skin (n = 48; 7), and whole blood (n = 44; 7) samples from serologically positive and negative dogs, respectively. Overall, the DNApol performed better than the Linj31 and LDON assays in the detection of positive samples in all tissues tested, yielding from 66.7 to 100.0% of positivity for both skin and spleen samples. For spleen samples, we observed no statistically significant differences between positive detection by the LDON and DNApol assays. Whole blood samples yielded the lowest rates of positive detection, regardless of the qPCR assay used. In contrast, positive detection of Leishmania DNA was as efficient from skin samples using the DNApol assay as from spleen samples using either the DNApol or the LDON assay. Although qPCR assays from skin samples may not be practical for use in the field, our study suggests that the DNApol and LDON assays from skin samples could be used in future to evaluate canine VL treatment in veterinary clinics.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Leishmania infantum / Doenças do Cão / Reação em Cadeia da Polimerase em Tempo Real / Leishmaniose Visceral Tipo de estudo: Diagnostic_studies Limite: Animals Idioma: En Revista: Parasitol Res Assunto da revista: PARASITOLOGIA Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Brasil País de publicação: Alemanha
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Leishmania infantum / Doenças do Cão / Reação em Cadeia da Polimerase em Tempo Real / Leishmaniose Visceral Tipo de estudo: Diagnostic_studies Limite: Animals Idioma: En Revista: Parasitol Res Assunto da revista: PARASITOLOGIA Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Brasil País de publicação: Alemanha