Multivariate curve resolution strategy for non-quadrilinear type 4 third-order/four way liquid chromatography-excitation-emission fluorescence matrix data.
Talanta
; 189: 509-516, 2018 Nov 01.
Article
em En
| MEDLINE
| ID: mdl-30086953
For the first time, a third-order/four-way system having instrumental modes depending on each other was experimentally generated and it was successfully resolved. Non-quadrilinear type 4 data, constituted by liquid chromatographic elution times (LC) and excitation-emission fluorescence matrices (EEFMs), were on-line measured using conventional equipment. Thus, third-order/four-way data, valuable for giving rise to highly sensitive and selective methods, were obtained minimizing significantly the experimental work and time, in comparison with the reported strategies for the acquisition of LC-EEFM data. The usefulness of MCR-ALS (multivariate curve resolution-alternating least square) for attaining reliable results from data with two mutually dependent instrumental modes, namely elution time and excitation wavelength modes, was established through the simultaneous quantitation of benz[a]anthracene, chrysene, benzo[b]fluoranthene, and benzo[a]pyrene. Limits of detection in the range 1.0-1.4â¯ngâ¯mL-1 were achieved for the target polycyclic aromatic hydrocarbons, allowing their determination in about 9â¯min per sample in leaves of different types of tea.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Revista:
Talanta
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
Argentina
País de publicação:
Holanda