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Unveiling the Multifaceted Mechanisms of Antibacterial Activity of Buforin II and Frenatin 2.3S Peptides from Skin Micro-Organs of the Orinoco Lime Treefrog (Sphaenorhynchus lacteus).
Muñoz-Camargo, Carolina; Salazar, Vivian A; Barrero-Guevara, Laura; Camargo, Sandra; Mosquera, Angela; Groot, Helena; Boix, Ester.
Afiliação
  • Muñoz-Camargo C; Department of Biomedical Engineering, Universidad de los Andes, Bogotá 111711, Colombia. c.munoz2016@uniandes.edu.co.
  • Salazar VA; Laboratorio de Genética Humana, Department of Biological Sciences, Universidad de los Andes, Bogotá 111711, Colombia. c.munoz2016@uniandes.edu.co.
  • Barrero-Guevara L; Laboratorio de Genética Humana, Department of Biological Sciences, Universidad de los Andes, Bogotá 111711, Colombia. visalazar@uniandes.edu.co.
  • Camargo S; Department of Biochemistry and Molecular Biology, Faculty of Biosciences, Universitat Autònoma de Barcelona, 08193 Cerdanyola del Vallès, Spain. visalazar@uniandes.edu.co.
  • Mosquera A; Laboratorio de Genética Humana, Department of Biological Sciences, Universidad de los Andes, Bogotá 111711, Colombia. la.barrero1854@uniandes.edu.co.
  • Groot H; Laboratorio de Genética Humana, Department of Biological Sciences, Universidad de los Andes, Bogotá 111711, Colombia. sandracamargouis@gmail.com.
  • Boix E; Institute of Life Sciences, Hebrew University of Jerusalem, Jerusalem 91904, Israel. sandracamargouis@gmail.com.
Int J Mol Sci ; 19(8)2018 Jul 25.
Article em En | MEDLINE | ID: mdl-30044391
Amphibian skin is a rich source of natural compounds with diverse antimicrobial and immune defense properties. Our previous studies showed that the frog skin secretions obtained by skin micro-organs from various species of Colombian anurans have antimicrobial activities against bacteria and viruses. We purified for the first time two antimicrobial peptides from the skin micro-organs of the Orinoco lime treefrog (Sphaenorhynchus lacteus) that correspond to Buforin II (BF2) and Frenatin 2.3S (F2.3S). Here, we have synthesized the two peptides and tested them against Gram-negative and Gram-positive bacteria, observing an effective bactericidal activity at micromolar concentrations. Evaluation of BF2 and F2.3S membrane destabilization activity on bacterial cell cultures and synthetic lipid bilayers reveals a distinct membrane interaction mechanism. BF2 agglutinates E. coli cells and synthetic vesicles, whereas F2.3S shows a high depolarization and membrane destabilization activities. Interestingly, we found that F2.3S is able to internalize within bacterial cells and can bind nucleic acids, as previously reported for BF2. Moreover, bacterial exposure to both peptides alters the expression profile of genes related to stress and resistance response. Overall, these results show the multifaceted mechanism of action of both antimicrobial peptides that can provide alternative tools in the fight against bacterial resistance.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Anuros / Proteínas / Peptídeos Catiônicos Antimicrobianos / Proteínas de Anfíbios / Antibacterianos Limite: Animals Idioma: En Revista: Int J Mol Sci Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Colômbia País de publicação: Suíça
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Anuros / Proteínas / Peptídeos Catiônicos Antimicrobianos / Proteínas de Anfíbios / Antibacterianos Limite: Animals Idioma: En Revista: Int J Mol Sci Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Colômbia País de publicação: Suíça