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Effect of antiprotozoal molecules on hypnospores of Perkinsus spp. parasite.
Duarte, Sâmia Sousa; de Moura, Ricardo Olímpio; da Silva, Patricia Mirella.
Afiliação
  • Duarte SS; Laboratório de Imunologia e Patologia de Invertebrados, Departamento de Biologia Molecular, Centro de Ciências Exatas e da Natureza, Universidade Federal da Paraíba -Campus I, 58051-900, João Pessoa, PB, Brazil. Electronic address: samiasduarte@gmail.com.
  • de Moura RO; Laboratório de Síntese e Vetorização de Moléculas, Departamento de Farmácia, Universidade Estadual da Paraíba, 58070-450, João Pessoa, PB, Brazil. Electronic address: ricardo.olimpiodemoura@gmail.com.
  • da Silva PM; Laboratório de Imunologia e Patologia de Invertebrados, Departamento de Biologia Molecular, Centro de Ciências Exatas e da Natureza, Universidade Federal da Paraíba -Campus I, 58051-900, João Pessoa, PB, Brazil. Electronic address: mirella_dasilva@hotmail.com.
Exp Parasitol ; 192: 25-35, 2018 Sep.
Article em En | MEDLINE | ID: mdl-30028986
Perkinsus protozoan parasites have been associated with high mortality of bivalves worldwide, including Brazil. The use of antiproliferative drugs to treat the Perkinsosis is an unusual prophylactic strategy. However, because of their environment impact it could be used to control parasite proliferation in closed system, such as hatchery. This study evaluated the anti-Perkinsus activity potential of synthesized and commercial compounds. Viability of hypnospores of Perkinsus spp. was assessed in vitro. Cells were incubated with three 2-amino-thiophene (6AMD, 6CN, 5CN) and one acylhydrazone derivatives (AMZ-DCL), at the concentrations of 31.25; 62.5; 125; 250 and 500 µM and one commercial chlorinated phenoxy phenol derivative, triclosan (2, 5, 10 and 20 µM), for 24-48 h. Two synthetic molecules (6CN and AMZ-DCL) caused a significant decline (38 and 39%, respectively) in hypnospores viability, at the highest concentration (500 µM), after 48 h. Triclosan was the most cytotoxic compound, causing 100% of mortality at 20 µM after 24 h and at 10 µM after 48 h. Cytotoxic effects of the compounds 6CN, AMZ-DCL, and triclosan were investigated by measuring parasite's zoosporulation, morphological changes and metabolic activities (esterase activity, production of reactive oxygen species and lipid content). Results showed that zoosporulation occurred in few cell. Triclosan caused changes in the morphology of hypnospores. The 6CN and AMZ-DCL did not alter the metabolic activities studied whilst Triclosan significantly increased the production of reactive oxygen species and changed the amount and distribution of lipids in the hypnospores. These results suggest that three compounds had potential to be used as antiprotozoal drugs, although further investigation of their mechanism of action must be enlightened.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ostreidae / Alveolados / Antiprotozoários Limite: Animals País/Região como assunto: America do sul / Brasil Idioma: En Revista: Exp Parasitol Ano de publicação: 2018 Tipo de documento: Article País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ostreidae / Alveolados / Antiprotozoários Limite: Animals País/Região como assunto: America do sul / Brasil Idioma: En Revista: Exp Parasitol Ano de publicação: 2018 Tipo de documento: Article País de publicação: Estados Unidos