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Photobiomodulation and different macrophages phenotypes during muscle tissue repair.
Souza, Nadhia H C; Mesquita-Ferrari, Raquel A; Rodrigues, Maria Fernanda S D; da Silva, Daniela F T; Ribeiro, Beatriz G; Alves, Agnelo N; Garcia, Mónica P; Nunes, Fábio D; da Silva Junior, Evaldo M; França, Cristiane M; Bussadori, Sandra K; Fernandes, Kristianne P S.
Afiliação
  • Souza NHC; Postgraduate Program in Rehabilition Sciences, Nove de Julho University (UNINOVE), São Paulo, Brazil.
  • Mesquita-Ferrari RA; Postgraduate Program in Rehabilition Sciences, Nove de Julho University (UNINOVE), São Paulo, Brazil.
  • Rodrigues MFSD; Postgraduate Program in Biophotonics Applied to Health Sciences, Nove de Julho University (UNINOVE), São Paulo, Brazil.
  • da Silva DFT; Postgraduate Program in Biophotonics Applied to Health Sciences, Nove de Julho University (UNINOVE), São Paulo, Brazil.
  • Ribeiro BG; Postgraduate Program in Biophotonics Applied to Health Sciences, Nove de Julho University (UNINOVE), São Paulo, Brazil.
  • Alves AN; Postgraduate Program in Rehabilition Sciences, Nove de Julho University (UNINOVE), São Paulo, Brazil.
  • Garcia MP; Postgraduate Program in Rehabilition Sciences, Nove de Julho University (UNINOVE), São Paulo, Brazil.
  • Nunes FD; Postgraduate Program in Biophotonics Applied to Health Sciences, Nove de Julho University (UNINOVE), São Paulo, Brazil.
  • da Silva Junior EM; Postgraduate Program in Biophotonics Applied to Health Sciences, Nove de Julho University (UNINOVE), São Paulo, Brazil.
  • França CM; Departament of Oral Pathology, School of Dentistry, University of São Paulo (FOUSP), São Paulo, Brazil.
  • Bussadori SK; Postgraduate Program in Biophotonics Applied to Health Sciences, Nove de Julho University (UNINOVE), São Paulo, Brazil.
  • Fernandes KPS; Postgraduate Program in Biophotonics Applied to Health Sciences, Nove de Julho University (UNINOVE), São Paulo, Brazil.
J Cell Mol Med ; 22(10): 4922-4934, 2018 10.
Article em En | MEDLINE | ID: mdl-30024093
Macrophages play a very important role in the conduction of several regenerative processes mainly due to their plasticity and multiple functions. In the muscle repair process, while M1 macrophages regulate the inflammatory and proliferative phases, M2 (anti-inflammatory) macrophages direct the differentiation and remodelling phases, leading to tissue regeneration. The aim of this study was to evaluate the effect of red and near infrared (NIR) photobiomodulation (PBM) on macrophage phenotypes and correlate these findings with the repair process following acute muscle injury. Wistar rats were divided into 4 groups: control; muscle injury; muscle injury + red PBM; and muscle injury + NIR PBM. After 2, 4 and 7 days, the tibialis anterior muscle was processed for analysis. Macrophages phenotypic profile was evaluated by immunohistochemistry and correlated with the different stages of the skeletal muscle repair by the qualitative and quantitative morphological analysis as well as by the evaluation of IL-6, TNF-α and TGF-ß mRNA expression. Photobiomodulation at both wavelengths was able to decrease the number of CD68+ (M1) macrophages 2 days after muscle injury and increase the number of CD163+ (M2) macrophages 7 days after injury. However, only NIR treatment was able to increase the number of CD206+ M2 macrophages (Day 2) and TGF-ß mRNA expression (Day 2, 4 and 7), favouring the repair process more expressivelly. Treatment with PBM was able to modulate the inflammation phase, optimize the transition from the inflammatory to the regeneration phase (mainly with NIR light) and improve the final step of regeneration, enhancing tissue repair.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Regeneração / Terapia com Luz de Baixa Intensidade / Desenvolvimento Muscular / Músculos Tipo de estudo: Qualitative_research Limite: Animals / Humans Idioma: En Revista: J Cell Mol Med Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Regeneração / Terapia com Luz de Baixa Intensidade / Desenvolvimento Muscular / Músculos Tipo de estudo: Qualitative_research Limite: Animals / Humans Idioma: En Revista: J Cell Mol Med Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido