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Paper-based enzymatic platform coupled to screen printed graphene-modified electrode for the fast neonatal screening of phenylketonuria.
Moreira, Cristian M; Pereira, Sirley V; Raba, Julio; Bertolino, Franco A; Messina, Germán A.
Afiliação
  • Moreira CM; INQUISAL, Departamento de Química, Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, Chacabuco 917, D5700BWS San Luis, Argentina.
  • Pereira SV; INQUISAL, Departamento de Química, Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, Chacabuco 917, D5700BWS San Luis, Argentina.
  • Raba J; INQUISAL, Departamento de Química, Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, Chacabuco 917, D5700BWS San Luis, Argentina.
  • Bertolino FA; INQUISAL, Departamento de Química, Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, Chacabuco 917, D5700BWS San Luis, Argentina.
  • Messina GA; INQUISAL, Departamento de Química, Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, Chacabuco 917, D5700BWS San Luis, Argentina. Electronic address: messina@unsl.edu.ar.
Clin Chim Acta ; 486: 59-65, 2018 Nov.
Article em En | MEDLINE | ID: mdl-30006287
INTRODUCTION: The PKU is an inborn error of amino acid metabolism, in which phenylalanine (Phe) accumulated in the blood causing alterations at the central nervous system. We report a novel paper-based enzymatic platform coupled to screen printed graphene-modified electrode for the neonatal screening of phenylketonuria (PKU0. METHODS: The paper-based analytical device coupled to electrochemical detection (EPAD) is based on the use of paper microzones modified with phenylalanine dehydrogenase enzyme (PheDH). The modified PADs were placed on the surface of an electrode modified with electrochemically reduced graphene (ERGO). PheDH in the presence of NAD+ catalyzes the reversible deamination of Phe to form phenylpyruvate, ammonia, and NADH. The electrochemical oxidation of NADH was monitored by differential pulse amperometry (DPA) at 0.6 V. The method was linear in the concentration range from 1 to 600 µmol/L of Phe with a LOQ of 1 µmol/L and LOD of 0.2 µmol/L. Within day precision was 5.7% across 3 levels of control samples. Between-day precision was 8.3%. The comparison with the standard Phe enzyme assay kit showed good agreement. The time required for the overall assay was <5 min. The non-sophisticated equipment required, the short assay time and the appropriate LOQ and LOD achieved by our EPAD make it an attractive and easy to use alternative compared to existing methods applied to the screening of PKU in neonatal samples.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Papel / Fenilcetonúrias / Triagem Neonatal / Aminoácido Oxirredutases / Grafite Tipo de estudo: Diagnostic_studies / Screening_studies Limite: Humans / Newborn Idioma: En Revista: Clin Chim Acta Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Argentina País de publicação: Holanda
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Papel / Fenilcetonúrias / Triagem Neonatal / Aminoácido Oxirredutases / Grafite Tipo de estudo: Diagnostic_studies / Screening_studies Limite: Humans / Newborn Idioma: En Revista: Clin Chim Acta Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Argentina País de publicação: Holanda