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Altered intestinal microbiota composition, antibiotic therapy and intestinal inflammation in children and adolescents with cystic fibrosis.
de Freitas, Maiara Brusco; Moreira, Emilia Addison Machado; Tomio, Camila; Moreno, Yara Maria Franco; Daltoe, Felipe Perozzo; Barbosa, Eliana; Ludwig Neto, Norberto; Buccigrossi, Vittoria; Guarino, Alfredo.
Afiliação
  • de Freitas MB; Graduate Program in Nutrition, Federal University of Santa Catarina, Florianópolis, Brazil.
  • Moreira EAM; Graduate Program in Nutrition, Federal University of Santa Catarina, Florianópolis, Brazil.
  • Tomio C; Graduate Program in Nutrition, Federal University of Santa Catarina, Florianópolis, Brazil.
  • Moreno YMF; Department of Nutrition, Graduate Program in Nutrition, Federal University of Santa Catarina, Florianópolis, Brazil.
  • Daltoe FP; Department of Pathology, Federal University of Santa Catarina, Florianópolis, Brazil.
  • Barbosa E; Joana de Gusmão Children´s Hospital, Santa Catarina, Florianópolis, Brazil.
  • Ludwig Neto N; Joana de Gusmão Children´s Hospital, Santa Catarina, Florianópolis, Brazil.
  • Buccigrossi V; Department of Translational Medical Science, Section of Pediatrics, University of Naples Federico II, Naples, Italy.
  • Guarino A; Department of Translational Medical Science, Section of Pediatrics, University of Naples Federico II, Naples, Italy.
PLoS One ; 13(6): e0198457, 2018.
Article em En | MEDLINE | ID: mdl-29933382
The aim of the present study was to evaluate the effect of cystic fibrosis and antibiotic therapy on intestinal microbiota composition and intestinal inflammation in children and adolescents. A cross-sectional controlled study was conducted with 36 children and adolescents: 19 in the cystic fibrosis group (CFG) and 17 in the control group (CG) matched for age and sex. The CFG was subdivided based on the use of antibiotic therapy (CFAB group) and non-use of antibiotic therapy (CFnAB group). The following data were evaluated: colonization, antibiotic therapy, mutation, breastfeeding, use of infant formula, type of delivery, introduction of solid foods, body mass index, fecal calprotectin and intestinal microbiota composition (fluorescence in situ hybridization). Intestinal inflammation evaluated by fecal calprotectin was significantly higher in the CFG (median: 40.80 µg/g, IQR: 19.80-87.10, p = 0.040) and CFAB group (median: 62.95 µg/g, IQR: 21.80-136.62, p = 0.045) compared to the CG (median: 20.15 µg/g, IQR: 16.20-31.00), and the Bacteroides, Firmicutes, Eubacterium rectale and Faecalibacterium prausnitzii were significantly decreased (p < 0.05) in the CFG compared to the CG, whereas the bacteria Clostridium difficile, Escherichia coli and Pseudomonas aeruginosa were significantly increased in the CFG (p < 0.05). The main differences were found between the CG and CFAB group for Eubacterium rectale (p = 0.006), Bifidobacterium (p = 0.017), Escherichia coli (p = 0.030), Firmicutes (p = 0.002), Pseudomonas aeruginosa (p < 0.001) and Clostridium difficile (p = 0.006). The results of this study confirm intestinal inflammation in patients with CF, which may be related to changes in the composition of the intestinal microbiota.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Bactérias / Fibrose Cística / Microbioma Gastrointestinal / Antibacterianos Tipo de estudo: Observational_studies / Prevalence_studies / Risk_factors_studies Limite: Child / Child, preschool / Female / Humans / Infant / Male Idioma: En Revista: PLoS One Assunto da revista: CIENCIA / MEDICINA Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
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XML
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Bactérias / Fibrose Cística / Microbioma Gastrointestinal / Antibacterianos Tipo de estudo: Observational_studies / Prevalence_studies / Risk_factors_studies Limite: Child / Child, preschool / Female / Humans / Infant / Male Idioma: En Revista: PLoS One Assunto da revista: CIENCIA / MEDICINA Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos