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Molecular and cellular effects of temperature in oysters Crassostrea brasiliana exposed to phenanthrene.
Lima, Daína; Zacchi, Flávia Lucena; Mattos, Jacó Joaquim; Flores-Nunes, Fabrício; Gomes, Carlos Henrique Araújo de Miranda; de Mello, Álvaro Cavaler Pessoa; Siebert, Marília Nardelli; Piazza, Clei Endrigo; Taniguchi, Satie; Sasaki, Silvio Tarou; Bícego, Márcia Caruso; Bebianno, Maria João; de Almeida, Eduardo Alves; Bainy, Afonso Celso Dias.
Afiliação
  • Lima D; Laboratory of Biomarkers of Aquatic Contamination and Immunochemistry, Department of Biochemistry, Federal University of Santa Catarina, UFSC, Florianópolis, SC, Brazil.
  • Zacchi FL; Laboratory of Biomarkers of Aquatic Contamination and Immunochemistry, Department of Biochemistry, Federal University of Santa Catarina, UFSC, Florianópolis, SC, Brazil.
  • Mattos JJ; Aquaculture Pathology Research, NEPAQ, Federal University of Santa Catarina, Florianópolis, SC, Brazil.
  • Flores-Nunes F; Laboratory of Biomarkers of Aquatic Contamination and Immunochemistry, Department of Biochemistry, Federal University of Santa Catarina, UFSC, Florianópolis, SC, Brazil.
  • Gomes CHAM; Laboratory of Marine Mollusks (LMM), Department of Aquaculture, Center of Agricultural Science, Federal University of Santa Catarina, Florianópolis, SC, Brazil.
  • de Mello ÁCP; Laboratory of Biomarkers of Aquatic Contamination and Immunochemistry, Department of Biochemistry, Federal University of Santa Catarina, UFSC, Florianópolis, SC, Brazil.
  • Siebert MN; Laboratory of Biomarkers of Aquatic Contamination and Immunochemistry, Department of Biochemistry, Federal University of Santa Catarina, UFSC, Florianópolis, SC, Brazil.
  • Piazza CE; Laboratory of Biomarkers of Aquatic Contamination and Immunochemistry, Department of Biochemistry, Federal University of Santa Catarina, UFSC, Florianópolis, SC, Brazil.
  • Taniguchi S; Laboratory of Marine Organic Chemistry, Oceanographic Institute, University of São Paulo, São Paulo, Brazil.
  • Sasaki ST; Laboratory of Marine Organic Chemistry, Oceanographic Institute, University of São Paulo, São Paulo, Brazil.
  • Bícego MC; Laboratory of Marine Organic Chemistry, Oceanographic Institute, University of São Paulo, São Paulo, Brazil.
  • Bebianno MJ; Laboratory of Biomarkers of Aquatic Contamination and Immunochemistry, Department of Biochemistry, Federal University of Santa Catarina, UFSC, Florianópolis, SC, Brazil; CIMA, University of Algarve, Campus de Gambelas, 8000-139 Faro, Portugal.
  • de Almeida EA; Department of Natural Sciences, Fundação Universidade Regional de Blumenau, Blumenau, SC, Brazil.
  • Bainy ACD; Laboratory of Biomarkers of Aquatic Contamination and Immunochemistry, Department of Biochemistry, Federal University of Santa Catarina, UFSC, Florianópolis, SC, Brazil. Electronic address: afonso.bainy@ufsc.br.
Chemosphere ; 209: 307-318, 2018 Oct.
Article em En | MEDLINE | ID: mdl-29933167
Exposure of aquatic organisms to polycyclic aromatic hydrocarbons (PAH), such as phenanthrene (PHE), may increase the production of reactive oxygen species (ROS) and cause changes in the biotransformation systems. In addition, changes in water temperature can cause adverse effects in the organisms. Estuarine species, like the oyster Crassostrea brasiliana, can adapt and tolerate temperature variation. To evaluate the influence of temperature on biological responses of C. brasiliana exposed to PHE, oysters were maintained at three temperatures (18, 24 and 32 °C) for 15 days and co-exposed afterwards to 100 µg.L-1 of PHE for 24 and 96 h. Levels of PHE in the water and oyster tissues were determined, respectively after 24 and 96 h. In addition, thermal stress, biotransformation and oxidative stress-related genes were analyzed in oyster gills, together with the activity of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione-S-transferases (GST) and levels of lipid peroxidation. Oyster accumulated significant levels of PHE. HSP70-like transcripts were affected by PHE exposure only at 32 °C. Transcript levels of cytochrome P450 isoforms (CYP2-like2 and CYP2AU1) were down-regulated in oysters exposed to PHE for 24 h at 32 °C. GSTΩ-like transcript levels were also down-regulated in the PHE-exposed group at 32 °C. After 96 h, CYP2-like2 transcripts were higher in the PHE exposed groups at 32 °C. Oysters kept at 18 °C showed higher levels of SOD-like transcripts, together with higher GST, GPx and G6PDH activities, associated to lower levels of lipoperoxidation. In general the biological responses evaluated were more affected by temperature, than by co-exposure to PHE.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fenantrenos / Poluentes Químicos da Água / Crassostrea Limite: Animals País/Região como assunto: America do sul / Brasil Idioma: En Revista: Chemosphere Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
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XML
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fenantrenos / Poluentes Químicos da Água / Crassostrea Limite: Animals País/Região como assunto: America do sul / Brasil Idioma: En Revista: Chemosphere Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido