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Effects of static magnetic fields on natural or magnetized mesenchymal stromal cells: Repercussions for magnetic targeting.
Silva, Luisa H A; Silva, Sueli M; Lima, Emilia C D; Silva, Renata C; Weiss, Daniel J; Morales, Marcelo M; Cruz, Fernanda F; Rocco, Patricia R M.
Afiliação
  • Silva LHA; Laboratory of Pulmonary Investigation, Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro, Av. Carlos Chagas Filho, 373, 21942-902, Rio de Janeiro, Rio de Janeiro, Brazil.
  • Silva SM; Institute of Chemistry, Federal University of Goiás, Av. Esperança, s/n, 74690-900, Goiânia, Goiás, Brazil.
  • Lima ECD; Institute of Chemistry, Federal University of Goiás, Av. Esperança, s/n, 74690-900, Goiânia, Goiás, Brazil.
  • Silva RC; National Institute of Metrology, Quality and Technology (Inmetro), Av Nossa Senhora das Graças, 50, 25250-020, Duque de Caxias, Rio de Janeiro, Brazil.
  • Weiss DJ; Department of Medicine, Vermont Lung Center, College of Medicine, University of Vermont, 89 Beaumont Ave. Given, Burlington, USA.
  • Morales MM; Laboratory of Cellular and Molecular Physiology, Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro, Av. Carlos Chagas Filho, 373, 21942-902, Rio de Janeiro, Rio de Janeiro, Brazil.
  • Cruz FF; Laboratory of Pulmonary Investigation, Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro, Av. Carlos Chagas Filho, 373, 21942-902, Rio de Janeiro, Rio de Janeiro, Brazil.
  • Rocco PRM; Laboratory of Pulmonary Investigation, Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro, Av. Carlos Chagas Filho, 373, 21942-902, Rio de Janeiro, Rio de Janeiro, Brazil. Electronic address: prmrocco@biof.ufrj.br.
Nanomedicine ; 14(7): 2075-2085, 2018 10.
Article em En | MEDLINE | ID: mdl-29933023
The magnetic targeting (MT) technique improves delivery of mesenchymal stromal cells (MSCs) to target sites. However, the moderate-intensity static magnetic fields (SMF) used for MT may exert adverse effects on MSCs. Thus, we aimed to evaluate the effects of SMF on MSCs in vitro. Cells were initially magnetized using citrate-coated magnetite nanoparticles. Then, control and magnetized MSCs were transferred to an in vitro MT system and exposed to 0.3-0.45 Tesla SMFs. MSC viability, morphology, ultrastructure, proliferation rates, differentiation, and immunomodulation were evaluated after 24 and 48 hours of exposure. MSCs temporarily lost viability and exhibited ultrastructural changes after exposure to SMFs, regardless of magnetization. Moreover, exposure to SMF reduced magnetized MSC proliferation rates. Nevertheless, MSCs remained functional (i.e., capable of differentiating, secreting repair mediators, and modulating alveolar macrophage phenotype). Thus, the experimental protocol tested in this experiment can be applied in future in vivo MT studies.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Diferenciação Celular / Macrófagos Alveolares / Proliferação de Células / Nanopartículas de Magnetita / Células-Tronco Mesenquimais / Campos Magnéticos Limite: Animals Idioma: En Revista: Nanomedicine Assunto da revista: BIOTECNOLOGIA Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Diferenciação Celular / Macrófagos Alveolares / Proliferação de Células / Nanopartículas de Magnetita / Células-Tronco Mesenquimais / Campos Magnéticos Limite: Animals Idioma: En Revista: Nanomedicine Assunto da revista: BIOTECNOLOGIA Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos