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Comparison between Ogawa-Kudoh and modified Petroff techniques for mycobacteria cultivation in the diagnosis of pulmonary tuberculosis.
Costa, Ronaldo Rodrigues da; Silva, Suzane Fernandes da; Fochat, Romário Costa; Macedo, Raquel Leite; Pereira, Thamiris Vilela; Silva, Marcio Roberto; Pinto, Carmen Perches Gomide; Leite, Isabel Cristina Gonçalves.
Afiliação
  • Costa RRD; Hospital Regional João Penido, Fundação Hospitalar do Estado de Minas Gerais,Juiz de Fora, MG, Brazil.
  • Silva SFD; Hospital Universitário, Universidade Federal de Juiz de Fora, Juiz de Fora, MG, Brazil.
  • Fochat RC; Hospital Universitário, Universidade Federal de Juiz de Fora, Juiz de Fora, MG, Brazil.
  • Macedo RL; Hospital Universitário, Universidade Federal de Juiz de Fora, Juiz de Fora, MG, Brazil.
  • Pereira TV; Hospital Universitário, Universidade Federal de Juiz de Fora, Juiz de Fora, MG, Brazil.
  • Silva MR; Hospital Universitário, Universidade Federal de Juiz de Fora, Juiz de Fora, MG, Brazil.
  • Pinto CPG; Empresa Brasileira de Pesquisa Agropecuária, Embrapa Gado de Leite, Juiz de Fora, MG, Brazil.
  • Leite ICG; Hospital Universitário, Universidade Federal de Juiz de Fora, Juiz de Fora, MG, Brazil.
Einstein (Sao Paulo) ; 16(2): eAO4214, 2018 Jun 11.
Article em En, Pt | MEDLINE | ID: mdl-29898027
OBJECTIVE: To compare the performance of the Ogawa-Kudoh method with the modified Petroff technique in diagnosis of pulmonary tuberculosis. METHODS: A total of 205 sputum samples from 166 patients with clinical suspicion or under pulmonary tuberculosis follow-up, seen at a public tertiary care hospital, from July 2014 to July 2016 were used. All samples were simultaneously processed using the Ogawa-Kudoh and modified Petroff decontamination methods, according to the recommendations of the Ministry of Health. In the statistical analysis, the McNemar test and the Kappa index were used, respectively, to compare proportions and verify agreement between data. RESULTS: The Ogawa-Kudoh and modified Petroff methods were efficient in mycobacteria detection, with no significant differences in results (p=0.549) and contamination rate of the cultures (p=0.065). The agreement between techniques was considered excellent (Kappa index of 0.877) and Ogawa-Kudoh, as compared to the modified Petroff technique, showed sensitivity of 90.4%, specificity of 96.6%, positive predictive value of 94.3% and negative predictive value of 94.2%. CONCLUSION: The Ogawa-Kudoh technique proved to be sufficiently sensitive and specific for diagnosis of pulmonary tuberculosis, and, therefore, suitable for routine laboratory application. Since it is simple, low-cost and has less technical requirements for biosafety and professional training, Ogawa-Kudoh is an alternative for managers and healthcare professionals to promote the expansion of bacteriological diagnostic coverage of pulmonary tuberculosis.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Escarro / Tuberculose Pulmonar / Técnicas Bacteriológicas / Mycobacterium Tipo de estudo: Diagnostic_studies / Guideline / Prognostic_studies Limite: Humans País/Região como assunto: America do sul / Brasil Idioma: En / Pt Revista: Einstein (Sao Paulo) Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Escarro / Tuberculose Pulmonar / Técnicas Bacteriológicas / Mycobacterium Tipo de estudo: Diagnostic_studies / Guideline / Prognostic_studies Limite: Humans País/Região como assunto: America do sul / Brasil Idioma: En / Pt Revista: Einstein (Sao Paulo) Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil