Erythrocyte acetylcholinesterase as biomarker of pesticide exposure: new and forgotten insights.

Assis, Caio R D; Linhares, Amanda G; Cabrera, Mariana P; Oliveira, Vagne M; Silva, Kaline C C; Marcuschi, Marina; Maciel Carvalho, Elba V M; Bezerra, Ranilson S; Carvalho, Luiz B

Assis, Caio R D; Linhares, Amanda G; Cabrera, Mariana P; Oliveira, Vagne M; Silva, Kaline C C; Marcuschi, Marina; Maciel Carvalho, Elba V M; Bezerra, Ranilson S; Carvalho, Luiz B.

Afiliação

Assis CRD; Laboratório de Enzimologia - LABENZ, Departamento de Bioquímica and Laboratório de Imunopatologia Keizo Asami - LIKA, Universidade Federal de Pernambuco, Recife, Pernambuco, 50670-901, Brazil. caiodias2@hotmail.com.
Linhares AG; Laboratório de Enzimologia - LABENZ, Departamento de Bioquímica and Laboratório de Imunopatologia Keizo Asami - LIKA, Universidade Federal de Pernambuco, Recife, Pernambuco, 50670-901, Brazil.
Cabrera MP; Laboratório de Enzimologia - LABENZ, Departamento de Bioquímica and Laboratório de Imunopatologia Keizo Asami - LIKA, Universidade Federal de Pernambuco, Recife, Pernambuco, 50670-901, Brazil.
Oliveira VM; Laboratório de Tecnologia de Produtos Bioativos, Departamento de Morfologia e Fisiologia Animal, DMFA, Universidade Federal Rural de Pernambuco, Recife, Pernambuco, Brazil.
Silva KCC; Laboratório de Enzimologia - LABENZ, Departamento de Bioquímica and Laboratório de Imunopatologia Keizo Asami - LIKA, Universidade Federal de Pernambuco, Recife, Pernambuco, 50670-901, Brazil.
Marcuschi M; Universidade Estadual da Bahia, Paulo Afonso, Bahia, Brazil.
Maciel Carvalho EVM; Laboratório de Enzimologia - LABENZ, Departamento de Bioquímica and Laboratório de Imunopatologia Keizo Asami - LIKA, Universidade Federal de Pernambuco, Recife, Pernambuco, 50670-901, Brazil.
Bezerra RS; Laboratório de Glicoproteínas, Departamento de Bioquímica, Universidade Federal de Pernambuco, Recife, PE, Brazil.
Carvalho LB; Laboratório de Enzimologia - LABENZ, Departamento de Bioquímica and Laboratório de Imunopatologia Keizo Asami - LIKA, Universidade Federal de Pernambuco, Recife, Pernambuco, 50670-901, Brazil.

Environ Sci Pollut Res Int ; 25(19): 18364-18376, 2018 Jul.

Article em En | MEDLINE | ID: mdl-29797194

RESUMO

Acetylcholinesterase (AChE) acts on the hydrolysis of acetylcholine, rapidly removing this neurotransmitter at cholinergic synapses and neuromuscular junctions as well as in neuronal growth and differentiation, modulation of cell adhesion ("electrotactins") and aryl-acylamidase activity (AAA). This enzyme is also found in erythrocyte, as 160 kDa dimer that anchors to the plasma membrane via glycophosphatidylinositol. The function of this enzyme in erythrocytes has not yet been elucidated; however, it is suspected to participate in cell-to-cell interactions. Here, a review on erythrocyte AChE characteristics and use as biomarker for organophosphorus and carbamate insecticides is presented since it is the first specific target/barrier of the action of these pesticides, besides plasma butyrylcholinesterase (BChE). However, some past and current methods have disadvantages: (a) not discriminating the activities of AChE and BChE; (b) low accuracy due to interference of hemoglobin in whole blood samples. On the other hand, extraction methods of hemoglobin-free erythrocyte AChE allows: (a) the freezing and transporting of samples; (b) samples free of colorimetric interference; (c) data from only erythrocyte AChE activity; (d) erythrocyte AChE specific activity presents higher correlation with the central nervous system AChE than other peripheral ChEs; (e) slow spontaneous regeneration against anti-ChEs agents of AChE in comparison to BChE, thus increasing the chances of detecting such compounds following longer interval after exposure. As monitoring perspectives, hemoglobin-free methodologies may be promising alternatives to assess the degree of exposure since they are not influenced by this interfering agent.

Assuntos

Acetilcolinesterase/sangue; Butirilcolinesterase/sangue; Exposição Ambiental/análise; Eritrócitos/enzimologia; Inseticidas/análise; Animais; Biomarcadores/sangue; Humanos

Palavras-chave

Aryl-acylamidase activity; Biomarker, acetylcholinesterase; Carbamates; Erythrocyte; Organophosphorus

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Acetilcolinesterase / Butirilcolinesterase / Exposição Ambiental / Eritrócitos / Inseticidas Limite: Animals / Humans Idioma: En Revista: Environ Sci Pollut Res Int Assunto da revista: SAUDE AMBIENTAL / TOXICOLOGIA Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Brasil País de publicação: Alemanha

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