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Comparison of GeneXpert MTB/RIF assay and LED-FM microscopy for the diagnosis of extra pulmonary tuberculosis in Khyber Pakhtunkhwa, Pakistan.
Khan, Anwar Sheed; Ali, Sajid; Khan, Muhammad Tahir; Ahmed, Sajjad; Khattak, Yasir; Irfan, Muhammad; Sajjad, Wasim.
Afiliação
  • Khan AS; Khyber Medical University Peshawar, Peshawar, Pakistan. Electronic address: Anwar786kp@hotmail.com.
  • Ali S; Provincial TB Reference Laboratory, Peshawar, Pakistan.
  • Khan MT; Capital University of Science & Technology, Islamabad, Pakistan.
  • Ahmed S; Khyber Medical University Peshawar, Peshawar, Pakistan.
  • Khattak Y; University of Peshawar, Centre for Biotechnology and Microbiology, Peshawar, Pakistan.
  • Abduljabbar; University of Haripur, Department of Medical Lab Technology, Haripur, Pakistan.
  • Irfan M; Sarhad University of Science and Information Technology, Institute of Biological Sciences, Peshawar, Pakistan.
  • Sajjad W; Department of Biogenetics, National University of Medical Sciences, Rawalpindi, Punjab, Pakistan.
Braz J Microbiol ; 49(4): 909-913, 2018.
Article em En | MEDLINE | ID: mdl-29752010
GeneXpert is one of the recent technological instruments used to diagnose tuberculosis in a short span of time. In this study, the performance of GeneXpert MTB/RIF assay for the diagnosis of extrapulmonary tuberculosis (EPTB) was compared with light-emitting diode Fluorescent Microscopy (LED-FM) in Khyber Pakhtunkhwa, Pakistan. A total of 737 EPTB samples were collected from tuberculosis (TB) suspected patients. Out of these samples, male to female ratio was 53% (n=390) to 47% (n=347) respectively. The sensitivity and specificity was 73% and 100% for GeneXpert, while 40% and 100% for LED-FM microscopy. This shows that the sensitivity of GeneXpert is 40-50%, higher than LED-FM microscopy. GeneXpert also detected low number of bacilli as compared to LED-FM microscopy.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Tuberculose Pulmonar / Reação em Cadeia da Polimerase / Reação em Cadeia da Polimerase em Tempo Real / Microscopia / Mycobacterium tuberculosis Tipo de estudo: Diagnostic_studies Limite: Adult / Female / Humans / Male / Middle aged País/Região como assunto: Asia Idioma: En Revista: Braz J Microbiol Ano de publicação: 2018 Tipo de documento: Article País de publicação: Brasil

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Tuberculose Pulmonar / Reação em Cadeia da Polimerase / Reação em Cadeia da Polimerase em Tempo Real / Microscopia / Mycobacterium tuberculosis Tipo de estudo: Diagnostic_studies Limite: Adult / Female / Humans / Male / Middle aged País/Região como assunto: Asia Idioma: En Revista: Braz J Microbiol Ano de publicação: 2018 Tipo de documento: Article País de publicação: Brasil