Caenorhabditis elegans Tolerates Hyperaccelerations up to 400,000 x g.
Astrobiology
; 18(7): 825-833, 2018 07.
Article
em En
| MEDLINE
| ID: mdl-29746159
One of the most important laboratory animal species is the nematode Caenorhabditis elegans, which has been used in a range of research fields such as neurobiology, body development, and molecular biology. The scientific progress obtained by employing C. elegans as a model in these areas has encouraged its use in new fields. One of the new potential applications concerns the biological responses to hyperacceleration stress (g-force), but only a few studies have evaluated the response of multicellular organisms to extreme hypergravity conditions at the order of magnitude 105 x g, which is the theorized force experienced by rocks ejected from Mars (or similar planets). Therefore, we subjected the nematode C. elegans to 400,000 x g (equivalent to that force) and evaluated viability, general morphology, and behavior of C. elegans after exposure to this stress. The metabolic activity of this nematode in response to the gravitational spectrum of 50-400,000 x g was also evaluated by means of the MTT assay. Surprisingly, we found that this organism showed no decrease in viability, no changes in behavior and development, and no drastic metabolic depression after hyperacceleration. Thus, we demonstrated for the first time that this multicellular research model can withstand extremely high g-forces, which prompts the use of C. elegans as a new model for extreme hypergravity. Key Words: Caenorhabditis elegans-Hypergravity-Ultracentrifugation-Acceleration-Panspermia-Astrobiology. Astrobiology 18, 825-833.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Estresse Fisiológico
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Caenorhabditis elegans
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Hipergravidade
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Exobiologia
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Aceleração
Limite:
Animals
Idioma:
En
Revista:
Astrobiology
Assunto da revista:
BIOLOGIA
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
Brasil
País de publicação:
Estados Unidos