BACKGROUND: This study aimed to verify the influence of different substrates with and without addition of nutrient solution on the roostocks production of Capdeboscq cultivar and grafted peach plants of 'Chimarrita' scion. METHODS: In the first experiment, the height and stem diameter of the rootstocks were evaluated every two weeks, up to 90 days after transplanting (DAT). At 90 DAT, dry weight of shoots and roots, total dry mass and Dickson Quality Index were evaluated. In the second experiment, seedlings of 'Capdeboscq' were grafted with 'Chimarrita' scion. The growth of the scions and the percentage of living grafts were evaluated. At 146 DAT, the stem diameter of the scions, the SPAD index, the chlorophyll and nitrogen balance index were evaluated. DISCUSSION: The greatest mean values for the stem diameter of seedlings of the cv. Capdeboscq were obtained with the substrates T4 (5.53 mm); T2 (5.47 mm) and T1 (5.23 mm) with addition of nutrient solution, with seedlings reaching the plant standards according to the ordinance number 173 of May 27th of 1984, which recommends that rootstocks have to have a minimum stem diameter of 5.0 mm. Thus, the substrates which received the addition of nutrient solution, except the soil substrate, were adequate considering the rules that governing the peach tree production in Brazilian Nurserioes. CONCLUSION: The nutrient solution already avaliblabe for Recent patents is highly indicated to obtain seedlings of 'Capbdboscq' rootstocks with high mophophysiological quality in less time during nursery cycle of plant production. The largest stem diameter for 'Capdeboscq' was obtained with substrate 35% sand + 15% soil + 50% bovine manure (5.53 mm); 75% sand + 25% soil (5.47 mm) and 100% sand (5.23 mm) with addition of nutrient solution. The best morphophysiological characteristics of 'Chimarrita' plants grafted on 'Capdeboscq' seedlings was obtained with the use of substrates 75% sand + 25% soil and 35% sand + 15% soil + 50% bovine manure, whose plants reached a morphological standard for sale, in just four and a half months DAT, that is 56 days after grafting.