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Automated detection of fluorescent cells in in-resin fluorescence sections for integrated light and electron microscopy.
Delpiano, J; Pizarro, L; Peddie, C J; Jones, M L; Griffin, L D; Collinson, L M.
Afiliação
  • Delpiano J; School of Engineering and Applied Sciences, Universidad de los Andes, Santiago, Chile.
  • Pizarro L; Department of Computer Science, University College London, London, United Kingdom.
  • Peddie CJ; Electron Microscopy, The Francis Crick Institute, London, United Kingdom.
  • Jones ML; Electron Microscopy, The Francis Crick Institute, London, United Kingdom.
  • Griffin LD; Department of Computer Science, University College London, London, United Kingdom.
  • Collinson LM; Electron Microscopy, The Francis Crick Institute, London, United Kingdom.
J Microsc ; 271(1): 109-119, 2018 07.
Article em En | MEDLINE | ID: mdl-29698565
Integrated array tomography combines fluorescence and electron imaging of ultrathin sections in one microscope, and enables accurate high-resolution correlation of fluorescent proteins to cell organelles and membranes. Large numbers of serial sections can be imaged sequentially to produce aligned volumes from both imaging modalities, thus producing enormous amounts of data that must be handled and processed using novel techniques. Here, we present a scheme for automated detection of fluorescent cells within thin resin sections, which could then be used to drive automated electron image acquisition from target regions via 'smart tracking'. The aim of this work is to aid in optimization of the data acquisition process through automation, freeing the operator to work on other tasks and speeding up the process, while reducing data rates by only acquiring images from regions of interest. This new method is shown to be robust against noise and able to deal with regions of low fluorescence.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Microscopia Eletrônica de Varredura / Proteínas / Microscopia de Fluorescência Tipo de estudo: Diagnostic_studies Limite: Humans Idioma: En Revista: J Microsc Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Chile País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Microscopia Eletrônica de Varredura / Proteínas / Microscopia de Fluorescência Tipo de estudo: Diagnostic_studies Limite: Humans Idioma: En Revista: J Microsc Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Chile País de publicação: Reino Unido