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Control of the adsorption properties of alginate - guar gum matrix functionalized with epichlorohydrin through the addition of different flexible chain polymers as toll for the chymotrypsinogen isolation.
Brassesco, M Emilia; Woitovich Valetti, Nadia; Picó, Guillermo A.
Afiliação
  • Brassesco ME; Institute of Biotechnological and Chemistry Processes, Faculty of Biochemical and Pharmaceutical Sciences and CONICET, National University of Rosario, Suipacha 570, S2002RLK Rosario, Argentina.
  • Woitovich Valetti N; Institute of Biotechnological and Chemistry Processes, Faculty of Biochemical and Pharmaceutical Sciences and CONICET, National University of Rosario, Suipacha 570, S2002RLK Rosario, Argentina.
  • Picó GA; Institute of Biotechnological and Chemistry Processes, Faculty of Biochemical and Pharmaceutical Sciences and CONICET, National University of Rosario, Suipacha 570, S2002RLK Rosario, Argentina. Electronic address: pico@iprobyq-conicet.gob.ar.
Int J Biol Macromol ; 115: 494-500, 2018 Aug.
Article em En | MEDLINE | ID: mdl-29678791
This work addresses the obtaining and characterization of alginate-guar gum matrix, cross-linked with epichlorohydrin in the presence of different flexible chain polymers: polyvinyl alcohol, polyvinyl pyrrolidine and Pluronic® F68. These matrixes were used for the adsorption of chymotrypsinogen and showed an increasing uptake in presence of the flexible chain polymer in the sense: none < Pluronic 68 < polyvinyl pyrrolidine < polyvinyl alcohol. The adsorption process was found to follow a first order kinetics model and was not influenced by the polymer type. It was found that Freundlich model was more suitable for our data. Polyvinyl alcohol and polyvinyl pyrrolidine addition increase the adsorption capacity of the original bed due to an increment in the rigidity of the gel caused by the formation of hydrogen bound between the polysaccharides and synthetics polymers.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Quimotripsinogênio / Alginatos / Epicloroidrina / Gomas Vegetais / Galactanos / Mananas Idioma: En Revista: Int J Biol Macromol Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Argentina País de publicação: Holanda

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Quimotripsinogênio / Alginatos / Epicloroidrina / Gomas Vegetais / Galactanos / Mananas Idioma: En Revista: Int J Biol Macromol Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Argentina País de publicação: Holanda