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Interlaboratory Comparison of Six Real-Time PCR Assays for Detection of Bovine Leukemia Virus Proviral DNA.
Jaworski, J P; Pluta, A; Rola-Luszczak, M; McGowan, S L; Finnegan, C; Heenemann, K; Carignano, H A; Alvarez, I; Murakami, K; Willems, L; Vahlenkamp, T W; Trono, K G; Choudhury, B; Kuzmak, J.
Afiliação
  • Jaworski JP; Instituto Nacional de Tecnología Agropecuaria (INTA), CICVyA, Instituto de Virología, Buenos Aires, Argentina jaworski.juan@inta.gob.ar.
  • Pluta A; Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Buenos Aires, Argentina.
  • Rola-Luszczak M; National Veterinary Research Institute (NVRI), Pulawy, Poland.
  • McGowan SL; National Veterinary Research Institute (NVRI), Pulawy, Poland.
  • Finnegan C; Animal and Plant Health Agency (APHA), Weybridge, United Kingdom.
  • Heenemann K; Animal and Plant Health Agency (APHA), Weybridge, United Kingdom.
  • Carignano HA; Institute of Virology, Center for Infectious Diseases, University of Leipzig, Leipzig, Germany.
  • Alvarez I; Instituto Nacional de Tecnología Agropecuaria (INTA), CICVyA, Instituto de Virología, Buenos Aires, Argentina.
  • Murakami K; Instituto Nacional de Tecnología Agropecuaria (INTA), CICVyA, Instituto de Virología, Buenos Aires, Argentina.
  • Willems L; Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Buenos Aires, Argentina.
  • Vahlenkamp TW; Department of Veterinary Medicine, Faculty of Agriculture, Iwate University, Morioka, Iwate, Japan.
  • Trono KG; Gembloux Agro-Bio Tech and GIGA Institute, University of Liège, Liège, Belgium.
  • Choudhury B; Institute of Virology, Center for Infectious Diseases, University of Leipzig, Leipzig, Germany.
  • Kuzmak J; Instituto Nacional de Tecnología Agropecuaria (INTA), CICVyA, Instituto de Virología, Buenos Aires, Argentina.
J Clin Microbiol ; 56(7)2018 07.
Article em En | MEDLINE | ID: mdl-29669790
Quantitative real-time PCR (qPCR) is increasingly being used for the detection of bovine leukemia virus (BLV) proviral DNA. Nevertheless, quality control for the validation and standardization of such tests is currently lacking. Therefore, the present study was initiated by three Office International des Epizooties (OIE) reference laboratories and three collaborating laboratories to measure the interlaboratory variability of six already developed and available BLV qPCR assays. For that purpose, an international panel of 58 DNA samples reflecting the dynamic range of the majority of the assays was distributed to six testing centers. Based on qualitative results, the overall agreement among all six laboratories was moderate. However, significant variability in the measurement of the BLV proviral DNA copy number was observed among different laboratories. Quantitative PCR assays, even when performed by experienced staff, can yield large variability in BLV proviral DNA copy numbers without harmonization. Further standardization of different factors (i.e., utilization of unified protocols and unique calibrators) should increase interlaboratory agreement.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Provírus / Leucose Enzoótica Bovina / Vírus da Leucemia Bovina / Carga Viral / Reação em Cadeia da Polimerase em Tempo Real Tipo de estudo: Diagnostic_studies / Qualitative_research Limite: Animals Idioma: En Revista: J Clin Microbiol Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Argentina País de publicação: Estados Unidos
Texto completo
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XML
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Provírus / Leucose Enzoótica Bovina / Vírus da Leucemia Bovina / Carga Viral / Reação em Cadeia da Polimerase em Tempo Real Tipo de estudo: Diagnostic_studies / Qualitative_research Limite: Animals Idioma: En Revista: J Clin Microbiol Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Argentina País de publicação: Estados Unidos