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Cyanotrophic and arsenic oxidizing activities of Pseudomonas mendocina P6115 isolated from mine tailings containing high cyanide concentration.
Miranda-Carrazco, Alejandra; Vigueras-Cortés, Juan M; Villa-Tanaca, Lourdes; Hernández-Rodríguez, César.
Afiliação
  • Miranda-Carrazco A; Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Prol. de Carpio y Plan de Ayala s/n. Col. Sto. Tomás, 11340, Cd. México, Mexico.
  • Vigueras-Cortés JM; Centro Interdisciplinario de Investigación para el Desarrollo Integral Regional-Unidad Durango, Instituto Politécnico Nacional, Sigma 119, Fraccionamiento 20 de Noviembre II, Dgo., Durango, 34220, Mexico.
  • Villa-Tanaca L; Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Prol. de Carpio y Plan de Ayala s/n. Col. Sto. Tomás, 11340, Cd. México, Mexico.
  • Hernández-Rodríguez C; Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Prol. de Carpio y Plan de Ayala s/n. Col. Sto. Tomás, 11340, Cd. México, Mexico. chdez38@hotmail.com.
Arch Microbiol ; 200(7): 1037-1048, 2018 Sep.
Article em En | MEDLINE | ID: mdl-29644379
Mine tailings and wastewater generate man-made environments with several selective pressures, including the presence of heavy metals, arsenic and high cyanide concentrations, but severe nutritional limitations. Some oligotrophic and pioneer bacteria can colonise and grow in mine wastes containing a low concentration of organic matter and combined nitrogen sources. In this study, Pseudomonas mendocina P6115 was isolated from mine tailings in Durango, Mexico, and identified through a phylogenetic approach of 16S rRNA, gyrB, rpoB, and rpoD genes. Cell growth, cyanide consumption, and ammonia production kinetics in a medium with cyanide as sole nitrogen source showed that at the beginning, the strain grew assimilating cyanide, when cyanide was removed, ammonium was produced and accumulated in the culture medium. However, no clear stoichiometric relationship between both nitrogen sources was observed. Also, cyanide complexes were assimilated as nitrogen sources. Other phenotypic tasks that contribute to the strain's adaptation to a mine tailing environment included siderophores production in media with moderate amounts of heavy metals, arsenite and arsenate tolerance, and the capacity of oxidizing arsenite. P. mendocina P6115 harbours cioA/cioB and aoxB genes encoding for a cyanide-insensitive oxidase and an arsenite oxidase, respectively. This is the first report where P. mendocina is described as a cyanotrophic and arsenic oxidizing species. Genotypic and phenotypic tasks of P. mendocina P6115 autochthonous from mine wastes are potentially relevant for biological treatment of residues contaminated with cyanide and arsenic.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Arsênio / Microbiologia do Solo / Cianetos / Pseudomonas mendocina País/Região como assunto: Mexico Idioma: En Revista: Arch Microbiol Ano de publicação: 2018 Tipo de documento: Article País de afiliação: México País de publicação: Alemanha
Texto completo
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Imprimir
XML
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Arsênio / Microbiologia do Solo / Cianetos / Pseudomonas mendocina País/Região como assunto: Mexico Idioma: En Revista: Arch Microbiol Ano de publicação: 2018 Tipo de documento: Article País de afiliação: México País de publicação: Alemanha