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Magnetic biocatalysts of pectinase and cellulase: Synthesis and characterization of two preparations for application in grape juice clarification.
Dal Magro, Lucas; Silveira, Vitória C C; de Menezes, Eliana Weber; Benvenutti, Edilson Valmir; Nicolodi, Sabrina; Hertz, Plinho F; Klein, Manuela P; Rodrigues, Rafael C.
Afiliação
  • Dal Magro L; Biotechnology, Bioprocess and Biocatalysis Group, Institute of Food Science and Technology, Federal University of Rio Grande do Sul, Av. Bento Gonçalves, 9500, P.O. Box 15090, ZC 91501-970 Porto Alegre, RS, Brazil.
  • Silveira VCC; Biotechnology, Bioprocess and Biocatalysis Group, Institute of Food Science and Technology, Federal University of Rio Grande do Sul, Av. Bento Gonçalves, 9500, P.O. Box 15090, ZC 91501-970 Porto Alegre, RS, Brazil.
  • de Menezes EW; Solids and Surfaces Laboratory, Institute of Chemistry, Federal University of Rio Grande do Sul, P.O. Box 15003, ZC 91501-970, Porto Alegre, RS, Brazil.
  • Benvenutti EV; Solids and Surfaces Laboratory, Institute of Chemistry, Federal University of Rio Grande do Sul, P.O. Box 15003, ZC 91501-970, Porto Alegre, RS, Brazil.
  • Nicolodi S; Magnetism Laboratory, Institute of Physics, Federal University of Rio Grande do Sul, P.O. Box 15051, ZC 91501-970 Porto Alegre, RS, Brazil.
  • Hertz PF; Biotechnology, Bioprocess and Biocatalysis Group, Institute of Food Science and Technology, Federal University of Rio Grande do Sul, Av. Bento Gonçalves, 9500, P.O. Box 15090, ZC 91501-970 Porto Alegre, RS, Brazil.
  • Klein MP; Department of Nutrition, Federal University of Health Sciences of Porto Alegre (UFCSPA), ZC 90050-170 Porto Alegre, RS, Brazil.
  • Rodrigues RC; Biotechnology, Bioprocess and Biocatalysis Group, Institute of Food Science and Technology, Federal University of Rio Grande do Sul, Av. Bento Gonçalves, 9500, P.O. Box 15090, ZC 91501-970 Porto Alegre, RS, Brazil. Electronic address: rafaelcrodrigues@ufrgs.br.
Int J Biol Macromol ; 115: 35-44, 2018 Aug.
Article em En | MEDLINE | ID: mdl-29634966
In the present study, we prepared two different magnetic biocatalysts of pectinase and cellulase: carrier-free magnetic CLEAs (CLEA-MP*) and immobilization on glutaraldehyde-activated magnetite (Enz-Glu-MP*). The biocatalysts were compared to their magnetic properties, immobilization parameters, stability and grape juice clarification. Enz-Glu-MP* presented higher magnetic properties than CLEA-MP*, whereas this presented higher surface area and pore volume. The KM of the enzyme immobilized on Enz-Glu-MP* was 25.65mM, lower in comparison to the CLEA-MP* (33.83mM). On the other hand, CLEA-MP* was the most active and stable biocatalyst, presenting higher recovered activity (33.4% of cellulase), higher thermal stability (2.39 stabilization factor) and improved reusability (8cycles). The integration of magnetic technology with enzymatic immobilization emerges as a possibility to increase the recover and reuse of biocatalysts for application in juice technology.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Poligalacturonase / Celulase / Vitis / Óxido Ferroso-Férrico / Sucos de Frutas e Vegetais Idioma: En Revista: Int J Biol Macromol Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Poligalacturonase / Celulase / Vitis / Óxido Ferroso-Férrico / Sucos de Frutas e Vegetais Idioma: En Revista: Int J Biol Macromol Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda