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HLA-G is expressed in intestinal samples of ulcerative colitis and Crohn's disease patients and HLA-G5 expression is differentially correlated with TNF and IL-10 cytokine expression.
Gomes, Renan Garcia; Brito, Carlos Alexandre Antunes de; Martinelli, Valéria Ferreira; Santos, Rossana Nascimento Dos; Gomes, Fabiana Oliveira Dos Santos; Peixoto, Christina Alves; Crispim, Janaína Oliveira; Diniz, George Tadeu Nunes; Donadi, Eduardo Antônio; Lucena-Silva, Norma.
Afiliação
  • Gomes RG; Aggeu Magalhães Institute, Oswaldo Cruz Foundation, Recife, Brazil.
  • Brito CAA; Department of Internal Medicine, School of Medicine, Federal University of Pernambuco, Recife, Brazil.
  • Martinelli VF; Department of Internal Medicine, School of Medicine, Federal University of Pernambuco, Recife, Brazil.
  • Santos RND; Aggeu Magalhães Institute, Oswaldo Cruz Foundation, Recife, Brazil.
  • Gomes FODS; Aggeu Magalhães Institute, Oswaldo Cruz Foundation, Recife, Brazil.
  • Peixoto CA; Aggeu Magalhães Institute, Oswaldo Cruz Foundation, Recife, Brazil.
  • Crispim JO; Department of Pharmacology, Federal University of Rio Grande do Norte, Natal, Brazil.
  • Diniz GTN; Aggeu Magalhães Institute, Oswaldo Cruz Foundation, Recife, Brazil.
  • Donadi EA; Department of Internal Medicine, Medical School of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil.
  • Lucena-Silva N; Aggeu Magalhães Institute, Oswaldo Cruz Foundation, Recife, Brazil. Electronic address: norma.lucena@hotmail.com.
Hum Immunol ; 79(6): 477-484, 2018 Jun.
Article em En | MEDLINE | ID: mdl-29588183
HLA-G is an immunomodulatory molecule that can be produced by epithelial cells. Considering that TNF and IL-10 participate in bowel inflammatory disorders and that both cytokines modulate HLA-G, we evaluated HLA-G, TNF and IL-10 mRNA expression by qPCR and HLA-G protein levels by immunohistochemistry in two intestinal samples exhibiting different degree of inflammation within a patient suffering from Crohn's disease (CD) or ulcerative colitis (UC). Tissue HLA-G5 (P < 0.0001), TNF (P = 0.0004) and IL-10 (P = 0.0169) mRNA expression levels were higher in intestinal areas exhibiting intense inflammation compared to areas of low inflammation, and HLA-G protein levels were not associated with degree of mucosal inflammation. In CD, the expression of TNF was correlated with IL-10 in low inflamed areas, exhibiting a TNF:IL-10 ratio = 3, but in inflamed areas the ratio increased to 9-folds. In UC, the expression of TNF was correlated to IL-10, irrespective of the inflammation grade, with little variation of the TNF:IL-10 ratio in the various inflamed areas. TNF and IL-10 expression was correlated with HLA-G5 expression in mild inflamed areas. Both CD and UC samples exhibited gene and protein expression of HLA-G; and the HLA-G5 expression is differentially correlated with TNF and IL-10 levels depending on the type of the underlying inflammatory bowel disorder.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Colite Ulcerativa / Doença de Crohn / Células Epiteliais / Antígenos HLA-G / Mucosa Intestinal Limite: Adolescent / Adult / Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Hum Immunol Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Colite Ulcerativa / Doença de Crohn / Células Epiteliais / Antígenos HLA-G / Mucosa Intestinal Limite: Adolescent / Adult / Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Hum Immunol Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos