Genome sequence of an aflatoxigenic pathogen of Argentinian peanut, Aspergillus arachidicola.

Moore, Geromy G; Mack, Brian M; Beltz, Shannon B; Puel, Olivier

Moore, Geromy G; Mack, Brian M; Beltz, Shannon B; Puel, Olivier.

Afiliação

Moore GG; Southern Regional Research Center, Agricultural Research Service, United States Department of Agriculture, 1100 Robert E Lee Blvd, New Orleans, Louisiana, 70124, USA. geromy.moore@ars.usda.gov.
Mack BM; Southern Regional Research Center, Agricultural Research Service, United States Department of Agriculture, 1100 Robert E Lee Blvd, New Orleans, Louisiana, 70124, USA.
Beltz SB; Southern Regional Research Center, Agricultural Research Service, United States Department of Agriculture, 1100 Robert E Lee Blvd, New Orleans, Louisiana, 70124, USA.
Puel O; Toxalim (Research Centre in Food Toxicology), Université de Toulouse, INRA, ENVT, INP-Purpan, UPS, Toulouse, France.

BMC Genomics ; 19(1): 189, 2018 03 09.

Article em En | MEDLINE | ID: mdl-29523080

RESUMO

BACKGROUND: Aspergillus arachidicola is an aflatoxigenic fungal species, first isolated from the leaves of a wild peanut species native to Argentina. It has since been reported in maize, Brazil nut and human sputum samples. This aflatoxigenic species is capable of secreting both B and G aflatoxins, similar to A. parasiticus and A. nomius. It has other characteristics that may result in its misidentification as one of several other section Flavi species. This study offers a preliminary analysis of the A. arachidicola genome. RESULTS: In this study we sequenced the genome of the A. arachidicola type strain (CBS 117610) and found its genome size to be 38.9 Mb, and its number of predicted genes to be 12,091, which are values comparable to those in other sequenced Aspergilli. A comparison of 57 known Aspergillus secondary metabolite gene clusters, among closely-related aflatoxigenic species, revealed nearly half were predicted to exist in the type strain of A. arachidicola. Of its predicted genes, 691 were identified as unique to the species and 60% were assigned Gene Ontology terms using BLAST2GO. Phylogenomic inference shows CBS 117610 sharing a most recent common ancestor with A. parasiticus. Finally, BLAST query of A. flavus mating-type idiomorph sequences to this strain revealed the presence of a single mating-type (MAT1-1) idiomorph. CONCLUSIONS: Based on A. arachidicola morphological, genetic and chemotype similarities with A. flavus and A. parasiticus, sequencing the genome of A. arachidicola will contribute to our understanding of the evolutionary relatedness among aflatoxigenic fungi.

Assuntos

Aflatoxinas/metabolismo; Aspergillus/genética; Genoma Fúngico; Sequenciamento de Nucleotídeos em Larga Escala/métodos; Transcriptoma; Arachis/microbiologia; Aspergillus/metabolismo; Proteínas Fúngicas/genética; Perfilação da Expressão Gênica; Ontologia Genética; Anotação de Sequência Molecular; Doenças das Plantas/microbiologia

Palavras-chave

Aspergillus arachidicola; Gene ontology; Genome sequence; Mating-type locus; Phylogenomics

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Aspergillus / Genoma Fúngico / Aflatoxinas / Sequenciamento de Nucleotídeos em Larga Escala / Transcriptoma Tipo de estudo: Prognostic_studies País/Região como assunto: America do sul / Argentina Idioma: En Revista: BMC Genomics Assunto da revista: GENETICA Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Estados Unidos País de publicação: Reino Unido

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