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An immunoproteomic approach revealing peptides from Sporothrix brasiliensis that induce a cellular immune response in subcutaneous sporotrichosis.
de Almeida, José Roberto Fogaça; Jannuzzi, Grasielle Pereira; Kaihami, Gilberto Hideo; Breda, Leandro Carvalho Dantas; Ferreira, Karen Spadari; de Almeida, Sandro Rogério.
Afiliação
  • de Almeida JRF; Faculty of Pharmaceutical Sciences, Department of Clinical and Toxicological Analysis, University of Sao Paulo, Sao Paulo, Brazil.
  • Jannuzzi GP; Faculty of Pharmaceutical Sciences, Department of Clinical and Toxicological Analysis, University of Sao Paulo, Sao Paulo, Brazil.
  • Kaihami GH; Institute of Chemistry, Department of Biochemistry, University of Sao Paulo, Sao Paulo, Brazil.
  • Breda LCD; Department of Immunology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
  • Ferreira KS; Department of Biological Sciences, Institute of Environmental, Chemical and Pharmaceutical Sciences, Federal University of São Paulo, Diadema, Brazil.
  • de Almeida SR; Faculty of Pharmaceutical Sciences, Department of Clinical and Toxicological Analysis, University of Sao Paulo, Sao Paulo, Brazil. sandroal@usp.br.
Sci Rep ; 8(1): 4192, 2018 03 08.
Article em En | MEDLINE | ID: mdl-29520092
Sporothrix brasiliensis is the most virulent fungus of the Sporothrix complex and is the main species recovered in the sporotrichosis zoonotic hyperendemic area in Rio de Janeiro. A vaccine against S. brasiliensis could improve the current sporotrichosis situation. Here, we show 3 peptides from S. brasiliensis immunogenic proteins that have a higher likelihood for engaging MHC-class II molecules. We investigated the efficiency of the peptides as vaccines for preventing subcutaneous sporotrichosis. In this study, we observed a decrease in lesion diameters in peptide-immunized mice, showing that the peptides could induce a protective immune response against subcutaneous sporotrichosis. ZR8 peptide is from the GP70 protein, the main antigen of the Sporothrix complex, and was the best potential vaccine candidate by increasing CD4+ T cells and higher levels of IFN-γ, IL-17A and IL-1ß characterizing a strong cellular immune response. This immune environment induced a higher number of neutrophils in lesions that are associated with fungus clearance. These results indicated that the ZR8 peptide induces a protective immune response against subcutaneous sporotrichosis and is a vaccine candidate against S. brasiliensis infection.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Peptídeos / Esporotricose / Sporothrix / Proteínas Fúngicas / Linfócitos T CD4-Positivos / Imunidade Celular Limite: Animals País/Região como assunto: America do sul / Brasil Idioma: En Revista: Sci Rep Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Peptídeos / Esporotricose / Sporothrix / Proteínas Fúngicas / Linfócitos T CD4-Positivos / Imunidade Celular Limite: Animals País/Região como assunto: America do sul / Brasil Idioma: En Revista: Sci Rep Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido